Supplementation of diets with glutamine and glutamic acid attenuated the effects of cold stress on intestinal mucosa and performance of weaned piglets

In this study we investigated the effect of glutamine and glutamic acid inclusion in the diet of weaned piglets subjected to cold stress and thermoneutral environment. Sixty-four weaned piglets were assessed from 28 to 65 days of age. A completely randomised design consisting of a 2 × 2 factorial arrangement was tested – environments (thermoneutral and cold stress) and diets (control and L-glutamine + L-glutamic acid (G + GA)). Performance, relative organ weight and carcass yield, and morphology of the intestinal mucosa were assessed. Supplementing the diets with G + GA reduced feed intake under both environments. This was associated with a decline in growth rate for piglets in the thermoneutral environment but not in the cold environment (P < 0.002). Feed efficiency was lower for piglets offered the control diets in the cold environment, but was significantly improved (24.6%) by G + GA supplementation in the cold but not the thermoneutral environment (P < 0.001). G + GA supplementation decreased small intestinal length and altered intestinal morphology with the highest villus/crypt depth ratio observed in piglets offered the G + GA supplemented diet in the cold environment. In summary, glutamine and glutamic acid diets mitigated the effects of cold stress on the intestinal mucosa and performance of weaned piglets.