[Effect of excretory/secretory protein of Trichinella spiralis adult worm on CLP-induced sepsis in mice].

To observe the effect of excretory/secretory products from Trichinella spiralis adult worms (AES) on cecal ligation and puncture (CLP) -induced sepsis in mice.Forty-eight BALB/c mice were randomly divided into 3 groups：a sham operation group (PBS + sham group, Group A), a CLP-induced sepsis group (PBS+CLP group, Group B) and an AES treatment group (AES+CLP group, Group C). The mice of each group were intraperitoneally injected with 25 μg of AES or PBS only as a control in a total volume of 200 μl. Eight mice from each group were selected randomly for survival analysis of 96 hours. The other 8 mice in each group were observed for pathological changes in the lung, liver and kidney tissues by HE staining 12 h after CLP, and then determined for the detection of cytokines including TNF-α, IL-1β, IL-6, IL-10 and TGF-β in the sera by ELISA.The difference among the survival rates of mice in the 3 groups was statistically significant (χ2 = 21.16, P < 0.05). Compared to Group A (100%), the survival rate of mice in Group B (0) decreased significantly (P < 0.05), and also the pathological damage degrees in the lung, liver and kidney tissues of the mice in Group B increased significantly after CLP. Compared with the mice in group B, the survival rate of those in Group C (70%) increased significantly (P < 0.05), and the pathological damage degrees in the lung, liver and kidney tissues of the mice in Group C decreased significantly after the treatment with AES. The differences among the levels of pro-inflammatory cytokines TNF-α (F = 27.11, P < 0.05), IL1β (F = 18.75, P < 0.05) and IL-6 (F = 100.93, P < 0.05) in the sera of the mice in the three groups were statistically significant. Compared with the mice in Group A, the levels of the 3 cytokines of those in Group B increased significantly (all P < 0.05). However, after the treatment with AES, the levels of the pro-inflammatory cytokines of those in Group C decreased significantly (all P < 0.05). The differences among the levels of immunoregulatory cytokines IL-10 (F = 10.88, P < 0.05) and TGF-β (F = 11.37, P < 0.05) in the sera of the mice in the three groups were also statistically significant. Compared with the mice in Group B, the levels of IL-10 and TGF-β of those in Group C were higher after treatment with AES (both P < 0.05).T. spiralis AES has a therapeutic potential for alleviating sepsis induced by CLP in mice.［摘要］目的 探讨旋毛虫成虫排泄分泌蛋白 (AES) 对盲肠结扎穿孔术 (CLP) 诱导的小鼠脓毒症的抑制作用。方法 将48只BALB/c小鼠随机分为3组, 即假手术对照组 (PBS + sham组, A组) 、脓毒症组 (PBS + CLP组, B组) 、AES蛋白治疗 组 (AES + CLP组, C组) 。A组和B组于CLP术后经腹腔注射200 μl PBS, C组术后经腹腔注射含25 μg AES蛋白的等量 PBS。每组随机抽取8只, 建模后观察各组小鼠的一般情况及96 h生存率; 每组其余8只小鼠于建模后12 h取肝、肺、肾, HE染色观察病理改变; 以酶联免疫吸附试验 (ELISA) 检测小鼠血清中TNF-α、IL-1β、IL-6、IL-10及TGF-β细胞因子水平 的变化。结果 3组小鼠96 h生存率差异有统计学意义 (χ2 = 21.16, P < 0.05); 与A组 (100%) 相比, B组小鼠生存率 (0) 降低 (P < 0.05), 且肺、肝及肾脏损伤明显加重; 经AES治疗后, C组存活率 (75%) 明显增加 (P < 0.05), 小鼠肺脏、肝脏及 肾脏病理损伤亦显著缓解。3组小鼠血清中的促炎因子TNF-α (F = 27.11, P < 0.05)、IL-1β (F = 18.75, P < 0.05)及IL-6 (F = 100.93, P < 0.05)水平差异均有统计学意义; B组小鼠的促炎因子水平均高于A组 (P 均< 0.05); 经AES治疗后, C组 小鼠上述3种促炎因子水平较B组均显著下降 (P 均< 0.05)。3组小鼠血清中的调节性细胞因子IL-10 (F = 10.88, P < 0.05)及TGF-β (F = 11.37, P < 0.05)水平差异亦有统计学意义; 经AES治疗后, C组小鼠IL-10及TGF-β分泌水平较B组亦 明显增加 (P < 0.05)。结论 旋毛虫AES对CLP诱导的BALB/c小鼠脓毒症有显著的缓解作用。.