生物传感器
石墨烯
材料科学
纳米技术
场效应晶体管
脑利钠肽
晶体管
生物标志物
心力衰竭
光电子学
医学
化学
内科学
电气工程
电压
工程类
生物化学
作者
Yongmin Lei,Mi Xiao,Yutao Li,Li Xu,Hong Zhang,Zhiyong Zhang,Guojun Zhang
标识
DOI:10.1016/j.bios.2016.12.018
摘要
Since brain natriuretic peptide (BNP) has become internationally recognized biomarkers in the diagnosis and prognosis of heart failure (HF), it is highly desirable to search for a novel sensing tool for detecting the patient's BNP level at the early stage. Here we report a platinum nanoparticles (PtNPs)-decorated reduced graphene oxide (rGO) field effect transistor (FET) biosensor coupled with a microfilter system for label-free and highly sensitive detection of BNP in whole blood. The PtNPs-decorated rGO FET sensor was obtained by drop-casting rGO onto the pre-fabricated FET chip and subsequently assembling PtNPs on the graphene surface. After anti-BNP was bound to the PtNPs surface, BNP was successfully detected by the anti-BNP immobilized FET biosensor. It was found that the developed FET biosensor was able to achieve a low detection limitation of 100 fM. Moreover, BNP was successfully detected in human whole blood sample treated by a custom-made microfilter, suggesting the sensor's capability of working in a complex sample matrix. The developed FET biosensor provides a new sensing platform for protein detection, showing its potential applications in clinic sample.
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