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Isolation and purification of six iridoid glycosides fromgardenia jasminoidesfruit by medium-pressure liquid chromatography combined with macroporous resin chromatography

色谱法 栀子花 化学 高效液相色谱法 环烯醚萜 逆流色谱法 柱色谱法 液相色谱-质谱法 质谱法 糖苷 有机化学 医学 病理 替代医学
作者
Yun Wang,Hui Liu,Lifeng Shen,Lan Yao,Yinlian Ma,Yu Dingrong,Jianhong Chen,Puling Li,Ying Chen,Cun Zhang
出处
期刊:Journal of Separation Science [Wiley]
卷期号:38 (23): 4119-4126 被引量:27
标识
DOI:10.1002/jssc.201500705
摘要

Gardeniae fructus is one of the most frequently used herbs in traditional Chinese medicine. In the present study, a process for the enrichment of six iridoid glycosides from Gardeniae fructus was developed using medium-pressure liquid chromatography combined with macroporous resin and reversed-phase chromatography. The purities of different fractions from Gardeniae fructus were assessed using quantitative high-performance liquid chromatography. After fractionation using HPD-100 column chromatography, a 30% ethanol fraction was selected based on high-performance liquid chromatography and liquid chromatography with mass spectrometry qualitative analysis to separate and purify. Based on the orientation analysis results, six compounds-deacetyl asperulosidic acid methyl ester, gardenoside, ixoroside, scandoside methyl ester, genipin-1-O-β-d-gentiobioside, and geniposide-were successfully isolated and purified in three to four combined steps from Gardeniae fructus. The purities of these compounds were found by high-performance liquid chromatography analysis to be 97.9, 98.1, 95.5, 96.3, 97.1, and 98.7%, respectively. Moreover, their structures were elucidated by NMR spectroscopy and liquid chromatography with tandem mass spectrometry. The separation process was highly efficient, rapid, and accurate, making it a potential approach for the large-scale production of iridoids in the laboratory and providing several marker compounds for quality control. This procedure may be meaningful for the purification of other natural products used in traditional Chinese medicine.

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