SH-SY5Y型
氧化应激
活性氧
化学
DNA损伤
硝基
脂质过氧化
细胞毒性
细胞凋亡
细胞内
超氧化物
活力测定
细胞生物学
生物化学
DNA
细胞培养
生物
神经母细胞瘤
光化学
体外
遗传学
酶
作者
Pennapa Chonpathompikunlert,Toru Yoshitomi,Jianzhong Han,Kazuko Toh,Hiroko Isoda,Yukio Nagasaki
出处
期刊:Therapeutic Delivery
[Newlands Press Ltd]
日期:2011-05-01
卷期号:2 (5): 585-597
被引量:21
摘要
Excessive accumulation of beta-amyloid (Abeta) has been proposed as a pivotal event in the pathogenesis of Alzheimer's disease. Possible mechanisms underlying Abeta-induced neuronal cytotoxicity include excess production of reactive oxygen species (ROS) and apoptosis. We have designed novel nanoparticles, nitroxyl radical-containing nanoparticles (RNPs), which possess nitroxyl radical in the core and chemically scavenges ROS. This study aimed to determine the potential neuroprotective role of RNPs on Abeta-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.SH-SY5Y cells were preincubated with 0.1-1 mM RNP for 24 h and then incubated with 20 microM Abeta1-42, for 48 h. In every group, cell viability, apoptotic rate, ROS levels including superoxide anion radicals and hydroxyl radicals, ROS production including lipid peroxidation, protein oxidation and DNA oxidation were measured.SH-SY5Y cells preincubated with 0.1-2 mM RNP for 24 h were protected from Abeta-induced damage. SH-SY5Y cells preincubated with more than 2 mM RNPfor 24 h showed cytotoxicity. From the quantitative analyses, it was observed that RNPs reduced intracellular oxidative stress. RNP treatment significantly reduced the amount of oxidized lipids, proteins and DNA. It also reduced DNA fragmentations, which caused lower apoptosis levels.RNPs are promising intracellular ROS scavengers.
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