T细胞受体
自然杀伤性T细胞
CD3型
分子生物学
转基因小鼠
生物
转基因
抗原
T细胞
细胞生物学
抗体
刺激
免疫学
化学
CD8型
免疫系统
内分泌学
生物化学
基因
作者
Michio Shimamura,Yiying Huang,Rika Migishima,Minesuke Yokoyama,Takako Saitoh,Takashi Yamamura
标识
DOI:10.1016/j.imlet.2008.08.002
摘要
Previously, we found that more than a half of the NK1.1+ T cell lines prepared from CD1−/− livers expressed invariant Vα19-Jα33 TCR α chains. Over-expression of the invariant Vα19-Jα33 TCR α transgene (Tg) with a natural TCR α promoter and an enhancer in mice induced the development of NK1.1+ T cells (Vα19 NKT cells) in the lymphoid organs, especially in the liver. Preferential usage of the Vα19 Tg by NKT cells in the transgenic mouse livers was indirectly indicated by the observation that few NK1.1+ TCRαβ+ cells of the Vα19 Tg livers were stained with a cocktail of anti-TCR Vα antibodies in the FACS analysis. Upon invariant TCR engagement in vivo following injection of mice with anti-CD3 antibody, NKT cells of the Tg mouse livers as well as spleens promptly produced immunoregulatory cytokines such as IL-4 and IFN-γ and altered surface receptor expression. Collectively, localization of Vα19 NKT cells in the liver is suggested that are ready to immediately response against antigen stimulation.
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