生物
转基因
基因
Cre-Lox重组
表型
基因剔除小鼠
Cre重组酶
胚胎干细胞
细胞生物学
功能(生物学)
遗传学
基因表达
基因表达调控
条件基因敲除
转基因小鼠
基因靶向
基因敲除
出处
期刊:Nutrition Reviews
[Oxford University Press]
日期:2004-06-01
卷期号:62 (6): 243-246
被引量:56
标识
DOI:10.1301/nr2004.jun243-246
摘要
Alteration of the mouse genome by conventional transgenic and gene-targeted approaches has greatly facilitated studies of gene function. However, a gene alteration expressed in the germ line may cause an embryonic lethal phenotype resulting in no viable mouse to study gene function. Similarly, a gene alteration may exert its effect in multiple different cell and tissue types, creating a complex phenotype in which it is difficult to distinguish direct function in a particular tissue from secondary effects resulting from altered gene function in other tissues. Therefore, methods have been developed to control conditions such as the timing, cell-type, and tissue specificity of gene activation or repression. This brief review provides an overview of the Cre/LoxP system for generating tissue-specific knockout mouse models.
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