乳酸脱氢酶
色谱法
化学
表皮葡萄球菌
糖尿病
检出限
酶
金黄色葡萄球菌
生物化学
医学
细菌
生物
内分泌学
遗传学
作者
Antony C. McLellan,Susan A. Phillips,Paul J. Thornalley
标识
DOI:10.1016/s0003-2697(05)80004-1
摘要
A fluorimetric assay for D-lactate in human blood samples was developed using an endpoint enzymatic assay with D-lactate dehydrogenase from Staphylococcus epidermidis. The intrabatch and interbatch coefficients of variance were 8.7% (n = 4) and 16.6% (n = 4), respectively. The limit of detection in blood was 3.73 nmol/ml. The assay suffers minor interference from S-D-lactoylglutathione, which was also present in the blood samples. The concentration of D-lactate in blood was (mean +/- SE, nmol/ml) normal healthy individuals, 11.0 +/- 1.2 (n = 7); and diabetic patients, 20.0 +/- 1.3 (n = 55) (a significant increase in diabetes mellitus; P < 0.01, Mann-Whitney U test).
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