微生物学
先天免疫系统
果蝇属(亚属)
细菌
黑腹果蝇
细菌外膜
受体
吞噬体
作者
Christine Kocks,Ju Hyun Cho,Nadine T. Nehme,Johanna Ulvila,Adam J. Pearson,Marie Meister,Charles M. Strom,Stephanie L. Conto,Charles Hétru,Lynda M. Stuart,Thilo Stehle,Jules A. Hoffmann,Jean‐Marc Reichhart,Dominique Ferrandon,Mika Rämet,R. A. B. Ezekowitz
出处
期刊:Cell
[Elsevier]
日期:2005-10-01
卷期号:123 (2): 335-346
被引量:362
标识
DOI:10.1016/j.cell.2005.08.034
摘要
Phagocytosis is a complex, evolutionarily conserved process that plays a central role in host defense against infection. We have identified a predicted transmembrane protein, Eater, which is involved in phagocytosis in Drosophila. Transcriptional silencing of the eater gene in a macrophage cell line led to a significant reduction in the binding and internalization of bacteria. Moreover, the N terminus of the Eater protein mediated direct microbial binding which could be inhibited with scavenger receptor ligands, acetylated, and oxidized low-density lipoprotein. In vivo, eater expression was restricted to blood cells. Flies lacking the eater gene displayed normal responses in NF-kappaB-like Toll and IMD signaling pathways but showed impaired phagocytosis and decreased survival after bacterial infection. Our results suggest that Eater is a major phagocytic receptor for a broad range of bacterial pathogens in Drosophila and provide a powerful model to address the role of phagocytosis in vivo.
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