OBJECTIVE To accurately define the anatomy of the neurovascular bundle (NVB) in men. MATERIALS AND METHODS The NVB was microdissected in detail bilaterally in 12 fixed human male adult cadavers. The anatomy of the NVB and its relationship to surrounding pelvic structures was ascertained in each specimen. RESULTS Previous reports of the anatomy of the NVB have not mentioned its levator ani and anterior rectal component. The anatomy of the cavernosal nerves is such that accurate graft anastomosis to proximal and distal cavernosal nerve segments is extremely difficult. CONCLUSION The current anatomical description of the cavernosal nerve and NVB is inaccurate.