血浆置换术
免疫吸附
抗体
红细胞压积
移植
纤维
化学
ABO血型系统
效价
色谱法
生物医学工程
材料科学
免疫学
医学
外科
内科学
复合材料
作者
Ahmad Alikhani,William J. Federspiel
摘要
Abstract Anti‐A/B antibody removal from blood in the peritransplantation period facilitates ABO‐incompatible transplantation and significantly increases the donor pool. We have been developing an anti‐A/B immunoadsorption device (BSAF), compatible with whole blood perfusion. The BSAF is based on integrated microfiltration hollow fibers with antibody capturing beads uniformly distributed within the fiber interstitial space. In this study we fabricated BSAF prototypes, appropriately scaled down from a conceptual clinical scale device. We then, for the first time, measured the time course of anti‐A capture from blood samples recirculating through the scaled down BSAF devices. We observed a significant reduction in IgM (96% ± 5%, n = 5, p < 0.001), and IgG (81% ± 18%, n = 5, p < 0.05) anti‐A antibody titers within 2 h. We did not observe a significant change between the initial and final values of hematocrit, total plasma protein concentration, plasma free hemoglobin concentration, and anti‐B antibody titer over five experiments. In conclusion we showed that the BSAF modules selectively removed anti‐A antibodies from blood in a simple one step process, without requiring a separate plasmapheresis unit. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
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