色谱法
检出限
化学
高效液相色谱法
土霉素
残留物(化学)
萃取(化学)
固相萃取
生物化学
抗生素
作者
Paulo Henrique M. Maia,Susanne Rath,Felix Guillermo Reyes Reyes
出处
期刊:Food Chemistry
[Elsevier]
日期:2008-07-01
卷期号:109 (1): 212-218
被引量:26
标识
DOI:10.1016/j.foodchem.2007.12.019
摘要
An analytical method for the determination of oxytetracycline (OTC) in tomatoes was developed and validated. Liquid-liquid extraction (LLE) and a solid-phase extraction (SPE) were used for sample preparation. Reversed-phase high performance liquid chromatography (RP-HPLC) using a C18 column and a mobile phase containing MeOH: calcium chloride, disodium ethylenediaminetetraacetate (EDTA) and sodium acetate, pH 7.3 (30:70, v/v), with fluorescence detection at 390nm excitation and 512nm emission, was used for separation and quantitation of OTC. The method was validated through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-day and inter-day precision, detection and quantitation limits and accuracy. Limit of quantitation show that the method developed is suitable for the determination of OTC at a level below the maximum residue limits established by the Brazilian legislations (250μgkg(-1)). Of 40 samples analyzed, none contained OTC above the limit of quantitation.
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