费斯特共振能量转移
荧光团
荧光
生命科学中的荧光
生物物理学
绿色荧光蛋白
化学
能量转移
纳米技术
生物
生物化学
材料科学
化学物理
物理
量子力学
基因
标识
DOI:10.1146/annurev-biochem-072909-094736
摘要
Many genetically encoded probes that employ fluorescent proteins and fluorescence resonance energy transfer (FRET) have been developed to better understand the spatiotemporal regulation of various cellular processes. The different types of FRET and measurement techniques necessitate characterization of their specific features. Here I provide theoretical and practical comparisons of bimolecular and unimolecular FRET constructs, intensity-based and lifetime-based FRET measurements, FRET imaging using live- and fixed-cell samples, green fluorescent protein-based and chemical fluorophore-based FRET, and FRET efficiency and indices. The potential benefits and limitations of a variety of features in the technologies using fluorescent proteins and FRET are discussed.
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