重组DNA
枯草芽孢杆菌
病毒学
免疫系统
生物
效价
病毒
抗体
微生物学
冠状病毒
猪流行性腹泻病毒
腹泻
接种疫苗
免疫学
医学
2019年冠状病毒病(COVID-19)
细菌
病理
传染病(医学专业)
内科学
基因
生物化学
疾病
遗传学
作者
Chunxiao Mou,Liqi Zhu,Xianping Xing,Jian Lin,Qian Yang
标识
DOI:10.1016/j.antiviral.2016.02.003
摘要
Transmissible gastroenteritis (TGE) causes severe diarrhea in suckling piglets, results in enormous economic loss in swine-producing areas of the world. To develop an effective, safe, and convenient vaccine for the prevention of TGE, we have constructed a recombinant Bacillus subtilis strain (B. subtilis CotGSG) displaying the transmissible gastroenteritis virus (TGEV) spike (S) protein and discussed its immune function to intestinal submucosal dendritic cells (DCs). Our results showed that the recombinant B. subtilis had the ability to recruit more DCs to sample B. subtilis CotGSG, migrate to MLNs, and induce immune responses. Immunized piglets with B. subtilis CotGSG could significantly elevate the specific SIgA titers in feces, IgG titers and neutralizing antibodies in serum. Collectively, our results suggested that recombinant B. subtilis CotGSG expressing the TGEV S protein could effectively induce immune responses via DCs, and provided a perspective on potential novel strategy and approach that may be applicable to the development of the next generation of TGEV vaccines.
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