清理
肝组织
骨小管
肝细胞
协议(科学)
染色
免疫荧光
病理
计算机科学
生物
医学
免疫学
内科学
生物化学
抗体
财务
经济
体外
替代医学
作者
Laura Molina,Yekaterina Krutsenko,Nathaniel Jenkins,Megan Smith,Junyan Tao,Travis B. Wheeler,Simon C. Watkins,Alan M. Watson,Satdarshan P. Monga
出处
期刊:STAR protocols
[Elsevier]
日期:2022-02-15
卷期号:3 (1): 101178-101178
被引量:4
标识
DOI:10.1016/j.xpro.2022.101178
摘要
Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation, decolorization, staining, and refractive index matching. LiverClear is sufficiently versatile to allow clearing of healthy and diseased mouse liver followed by immunofluorescence staining and imaging to visualize intact 3D structures such as bile ducts and hepatocyte canaliculi. We also adapted this protocol for clearing human livers. For complete details on the use and execution of this protocol, please refer to Molina et al. (2021).
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