An hiPSC-Derived In Vitro Model of the Blood–Brain Barrier

Human induced pluripotent stem cells (hiPSC)Human induced pluripotent stem cells (hiPSC) offer a tractable system to model the blood–brainBrains barrierBarriers (BBBBlood-brain barrier (BBB)). Here we detail the assembly of a triple co-cultureCo-culture hiPSC-BBB model, using hiPSC-derived brain microvascular endothelial cellsBrain microvascular endothelial cells (BMECs) (BMECBrain microvascular endothelial cell (BMEC)), astrocytes, and mural cells (MC). Transendothelial electrical resistance (TEER)Transendothelial electrical resistance (TEER) and sodium fluorescein (NaFl) permeability can be used to test the barrierBarriers properties. The model has applications in studying BBB-related pathology and for drug screeningPermeabilityscreening.