Ethanol extract of propolis protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting CD36 expression and endoplasmic reticulum stress-C/EBP homologous protein pathway

内质网 CD36 蜂胶 细胞凋亡 未折叠蛋白反应 医学 细胞生物学 蛋白质表达 生物化学 化学 传统医学 生物 基因
作者
Hua Tian,Hong‐Wei Sun,Jiajun Zhang,Xiaowei Zhang,Li Zhao,Shoudong Guo,Yanyan Li,Peng Jiao,Hao Wang,Shucun Qin,Shutong Yao
出处
期刊:BMC Complementary and Alternative Medicine [BioMed Central]
卷期号:15 (1) 被引量:20
标识
DOI:10.1186/s12906-015-0759-4
摘要

Ethanol extract of propolis (EEP), rich in flavones, has been known for various biological activities including antioxidant, antiinflammatory and antibiotic activities. Our previous studies have shown that EEP protects endothelial cells from oxidized low-density lipoprotein (ox-LDL)-induced apoptosis and inhibits atherosclerotic lesion development. In this present study, we explored the protective effect of EEP on ox-LDL-induced cytotoxicity in macrophages and specifically the endoplasmic reticulum (ER) stress-C/EBP homologous protein (CHOP) pathway-mediated apoptosis. EEP was prepared and the total flavonoids content of EEP was determined by the colorimetric method of Chinese Standard (GB/T 20574-2006). The effects of EEP on lipid accumulation, cytotoxicity and apoptosis in RAW264.7 cells induced by ox-LDL or tunicamycin (TM, an ER stress inducer) were assayed using oil red O staining, MTT assay, flow cytometric analysis and so on. Immunofluorescence, Western blot and real time-PCR analysis were then used to further investigate the molecular mechanisms by which EEP protects macrophages from ox-LDL-induced apoptosis. 4-phenylbutyric acid (PBA), an ER stress inhibitor, was used as a positive control. EEP (7.5, 15 and 30 mg/L) not only attenuated ox-LDL-induced lipid accumulation in RAW264.7 macrophages in a dose-dependent manner but also inhibited the decreased cell viability and the increased lactate dehydrogenase (LDH) leakage, caspase-3 activation and apoptosis induced by ox-LDL or tunicamycin (TM, a classical ER stress inducer), which were similar to 4-phenylbutyric acid (PBA, an inhibitor of ER stress) treatment. In addition, like PBA, EEP significantly suppressed the ox-LDL- or TM-induced activation of ER stress signaling pathway including the phosphorylation of double-stranded RNA-activated protein kinase-like ER kinase (PERK) and eukaryotic translation initiation factor 2α (eIF2α) as well as upregulation of glucose regulated protein 78 (GRP78) and the pro-apoptotic protein CHOP. Furthermore, EEP significantly suppressed ox-LDL intake by macrophages and the upregulation of CD36 induced by ox-LDL. These data indicate that EEP may protect macrophages from ox-LDL-induced apoptosis and the mechanism at least partially involves its ability to suppress the CD36-mediated ox-LDL intake and subsequent activation of ER stress-CHOP signalling pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
小胖熊完成签到,获得积分10
1秒前
1秒前
bgt发布了新的文献求助10
2秒前
张灬小胖完成签到,获得积分10
2秒前
Mmm发布了新的文献求助10
2秒前
星辰大海应助hhh采纳,获得10
2秒前
2秒前
2秒前
3秒前
3秒前
3秒前
joni完成签到,获得积分10
3秒前
111完成签到,获得积分10
4秒前
会走路的番茄完成签到,获得积分10
4秒前
汉堡包应助闪闪的梦柏采纳,获得10
4秒前
可爱的函函应助菠菜采纳,获得200
4秒前
5秒前
Jenny_Zhan完成签到,获得积分10
5秒前
6秒前
JoshuaChen发布了新的文献求助10
6秒前
火星上香菇完成签到,获得积分10
7秒前
7秒前
暮歌发布了新的文献求助50
7秒前
8秒前
迷路念真完成签到,获得积分20
8秒前
Jenny_Zhan发布了新的文献求助10
8秒前
可耐的发夹完成签到 ,获得积分10
8秒前
fang发布了新的文献求助10
9秒前
爆米花完成签到,获得积分10
9秒前
ZZZ发布了新的文献求助10
9秒前
NexusExplorer应助bgt采纳,获得30
10秒前
11秒前
喜之郎完成签到,获得积分10
11秒前
自然1111发布了新的文献求助10
11秒前
牛姐发布了新的文献求助20
11秒前
迷路念真发布了新的文献求助10
11秒前
JETSTREAM完成签到,获得积分10
12秒前
13秒前
靓丽安珊发布了新的文献求助10
13秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Handbook of Marine Craft Hydrodynamics and Motion Control, 2nd Edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3987078
求助须知:如何正确求助?哪些是违规求助? 3529488
关于积分的说明 11245360
捐赠科研通 3267987
什么是DOI,文献DOI怎么找? 1804013
邀请新用户注册赠送积分活动 881270
科研通“疑难数据库(出版商)”最低求助积分说明 808650