Isolation and purification of food‐grade C‐phycocyanin from Arthrospira platensis and its determination in confectionery by HPLC with diode array detection

藻蓝蛋白 色谱法 藻胆蛋白 化学 螺旋藻(膳食补充剂) 萃取(化学) 高效液相色谱法 藻蓝蛋白 颜料 节螺藻属 蓝藻 生物 细菌 有机化学 遗传学 原材料
作者
Maria Kissoudi,Ioannis Sarakatsianos,Victoria Samanidou
出处
期刊:Journal of Separation Science [Wiley]
卷期号:41 (4): 975-981 被引量:50
标识
DOI:10.1002/jssc.201701151
摘要

Abstract C‐Phycocyanin is the major phycobiliprotein in Arthrospira platensis , also known as Spirulina , which is a cyanobacterium used as a dietary supplement because of its powerful effects on body and brain. C‐phycocyanin is a blue‐colored accessory photosynthetic pigment with multiple applications in food industry as natural dye or additive, and in pharmaceuticals. This study presents a simple protocol for the extraction and purification of food‐grade C‐phycocyanin from Arthrospira platensis . The cell lysis of cyanobacterium was performed by sonication combined with repeated freezing and thawing cycles. The purification of the crude extract of C‐phycocyanin was carried out by ammonium sulfate precipitation followed by ion exchange chromatography resulting in 2.5 purity. The purity of phycocyanobilin chromophore has been tested by UV‐visible spectrophotometry by monitoring the absorption after each stage of purification. A high‐performance liquid chromatography method has been developed and validated for the determination of food‐grade C‐phycocyanin. Intra‐ and interday precision values less than 5.6% and recovery greater than 91.2% indicated high precision and accuracy of the method for analysis of C‐phycocyanin. The method has been applied to commercial confectionery of blue color and to the purified protein obtained in the first stage of the study.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
寂寞的朋友完成签到,获得积分10
1秒前
1秒前
ding应助agleam采纳,获得30
2秒前
田様应助有魅力的芷文采纳,获得10
2秒前
我是老大应助wendydqw采纳,获得10
2秒前
2秒前
2秒前
LonelyCMA完成签到 ,获得积分10
3秒前
谢亚飞完成签到,获得积分20
3秒前
一米阳光完成签到,获得积分10
3秒前
一颗橙子完成签到,获得积分10
4秒前
江上清风游完成签到,获得积分0
4秒前
天tian完成签到,获得积分10
6秒前
GGbond发布了新的文献求助10
6秒前
Andrew完成签到,获得积分10
6秒前
虚心的山柏完成签到,获得积分10
6秒前
ZHANG_Kun发布了新的文献求助80
6秒前
susu完成签到,获得积分10
7秒前
yolo完成签到,获得积分10
7秒前
7秒前
baijiayi完成签到,获得积分10
8秒前
IBMffff完成签到,获得积分0
9秒前
xinqisusu完成签到,获得积分10
9秒前
wst1988完成签到,获得积分10
9秒前
9秒前
王一完成签到,获得积分10
9秒前
一路向北4956完成签到,获得积分0
10秒前
热情的幻丝完成签到,获得积分20
10秒前
10秒前
胡不喜完成签到,获得积分10
11秒前
susu发布了新的文献求助20
11秒前
高贵芷天发布了新的文献求助10
11秒前
栗子完成签到,获得积分10
11秒前
超人不会飞完成签到,获得积分10
13秒前
13秒前
Akim应助lvyan采纳,获得10
14秒前
YANA完成签到,获得积分10
14秒前
西念花完成签到,获得积分20
14秒前
能干睫毛发布了新的文献求助10
15秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 850
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3249108
求助须知:如何正确求助?哪些是违规求助? 2892426
关于积分的说明 8271779
捐赠科研通 2560741
什么是DOI,文献DOI怎么找? 1389228
科研通“疑难数据库(出版商)”最低求助积分说明 651047
邀请新用户注册赠送积分活动 627873