下胚轴
光敏色素
赤霉素
拟南芥
细胞生物学
突变体
拟南芥
生物
光遗传学
调节器
生物物理学
植物
生物化学
基因
神经科学
红灯
作者
Annalisa Rizza,Ankit Walia,Viviane Lanquar,Wolf B. Frommer,Alexander M. Jones
出处
期刊:Nature plants
[Springer Nature]
日期:2017-09-29
卷期号:3 (10): 803-813
被引量:152
标识
DOI:10.1038/s41477-017-0021-9
摘要
The phytohormone gibberellin (GA) is a key regulator of plant growth and development. Although the upstream regulation and downstream responses to GA vary across cells and tissues, developmental stages and environmental conditions, the spatiotemporal distribution of GA in vivo remains unclear. Using a combinatorial screen in yeast, we engineered an optogenetic biosensor, GIBBERELLIN PERCEPTION SENSOR 1 (GPS1), that senses nanomolar levels of bioactive GAs. Arabidopsis thaliana plants expressing a nuclear localized GPS1 report on GAs at the cellular level. GA gradients were correlated with gradients of cell length in rapidly elongating roots and dark-grown hypocotyls. In roots, accumulation of exogenously applied GA also correlated with cell length, intimating that a root GA gradient can be established independently of GA biosynthesis. In hypocotyls, GA levels were reduced in a phytochrome interacting factor (pif) quadruple mutant in the dark and increased in a phytochrome double mutant in the light, indicating that PIFs elevate GA in the dark and that phytochrome inhibition of PIFs could lower GA in the light. As GA signalling directs hypocotyl elongation largely through promoting PIF activity, PIF promotion of GA accumulation represents a positive feedback loop within the molecular framework driving rapid hypocotyl growth.
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