ToxR modulates biofilm formation in fish pathogen Vibrio harveyi

哈维氏弧菌 生物 微生物学 生物膜 毒力 病菌 基因 突变体 弧菌 细菌 遗传学
作者
Yingying Zhang,Deng Yan-Chun,Juan Feng,Jun Hu,Haoxiang Chen,Zhenhua Guo,Ruochun Gao,Yingying Su
出处
期刊:Letters in Applied Microbiology [Wiley]
卷期号:74 (2): 288-299 被引量:6
标识
DOI:10.1111/lam.13606
摘要

Vibrio harveyi is a common aquaculture pathogen causing diseases in a variety of aquatic animals. toxR, a conserved virulence-associated gene in vibrios, is identified in V. harveyi 345, a pathogenic strain isolated from diseased fish. In this study, to gain insight into function of ToxR in V. harveyi, an in-frame deletion of the toxR gene was constructed to reveal the role of ToxR in the physiology and virulence of V. harveyi. The statistical analysis showed no significant differences in the growth ability, motility, extracellular protease secretion, antibiotic susceptibility, virulence by intraperitoneal injection and the ability of V. harveyi to colonize the spleen and liver tissues of the pearl gentian grouper between the wild-type (WT) and the toxR mutant. However, the deletion of toxR increased the biofilm formation. The structure of the V. harveyi biofilm was further analysed by using scanning electron microscopy (SEM) and confocal laser scanning microscopy, and the results showed that deletion of toxR increased the number and density of V. harveyi biofilm. Since biofilm production is flagella, exopolysaccharide (EPS) and lipopolysaccharide dependent, 16 of V. harveyi biofilm-related genes were selected for further analysis. Based on quantitative real-time reverse transcription-PCR, the expression levels of these genes, including genes flrB, motY and mshA, flaE, flrA and gmhD, were significantly up-regulated in the ΔtoxR+ strain as compared with the WT+ and C-ΔtoxR strains during the early and mid-exponential, while epsG, flaA, flaE, flgD, flgE, flrB, flrC, lpxB, motY, mshA and scrG genes were inhibited because of deletion of the toxR gene in the stationary growth phase. Our results indicate that ToxR plays an important role in controlling the biofilm in V. harveyi.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
爆米花应助易寒采纳,获得10
1秒前
科研通AI2S应助mm采纳,获得10
1秒前
所所应助Rian采纳,获得10
2秒前
iY完成签到 ,获得积分10
3秒前
3秒前
4秒前
4秒前
Cassie发布了新的文献求助20
5秒前
FashionBoy应助冷傲初夏采纳,获得10
6秒前
yixin发布了新的文献求助10
6秒前
9秒前
上官若男应助听弦采纳,获得10
11秒前
MalowZhang发布了新的文献求助10
12秒前
乐乐乐乐乐乐应助牧歌采纳,获得10
12秒前
zhaoxicun发布了新的文献求助30
13秒前
15秒前
JamesPei应助小孙采纳,获得10
15秒前
15秒前
大模型应助范医生01采纳,获得10
16秒前
18秒前
李李李发布了新的文献求助10
18秒前
调皮飞雪发布了新的文献求助10
18秒前
19秒前
wjc完成签到,获得积分20
20秒前
今后应助ruanruan采纳,获得10
21秒前
开放衣发布了新的文献求助10
22秒前
Eraser完成签到,获得积分10
23秒前
Fourteen完成签到,获得积分10
23秒前
24秒前
酷波er应助cabbage008采纳,获得10
24秒前
仙茅应助linn采纳,获得100
25秒前
Zhou发布了新的文献求助10
25秒前
26秒前
千纸鹤发布了新的文献求助10
27秒前
端庄子轩发布了新的文献求助10
27秒前
28秒前
28秒前
小蘑菇应助华开放采纳,获得10
28秒前
调皮飞雪完成签到,获得积分10
28秒前
28秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 870
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3256323
求助须知:如何正确求助?哪些是违规求助? 2898596
关于积分的说明 8301615
捐赠科研通 2567759
什么是DOI,文献DOI怎么找? 1394681
科研通“疑难数据库(出版商)”最低求助积分说明 652913
邀请新用户注册赠送积分活动 630557