Start-up of Anammox systems with different biochar amendment: Process characteristics and microbial community

As the ‘go-to’ process when it comes to biological nitrogen removal from wastewaters in recent years, the Anammox process has undergone lots of investigations in order to optimize its performance. In evaluating the effect of distinct biochar types at different concentrations on the Anammox startup process, as well as analyze their corresponding influence on the microbial community structure, three additives (coconut, peach, and bamboo) at either 5%, 10%, or 15% respectively were amended in various Anammox EGSB setups. (i). The 5% coconut biochar amendment resulted in the fastest startup of 46 days with an average ammonium removal efficiency of 96% whereas the control setup took 69 days. Thus, a more robust and cost effective Anammox process could be realized on an industrial scale. (ii) The Illumina high-throughput sequencing of the collected sludge samples indicated that the amendment with distinct biochar resulted in varied prevailing microbial communities in the respective setups. (iii) Proteobacteria was the dominant microbial community. (iv) However, two Anammox bacteria species, Candidatus Brocadia and Candidatus Jettenia were identified, with relative abundances of 0–4.72% and 0–6.23% respectively. The results from this study illustrate the correlation between Anammox reactor performance (startup and nitrogen removal efficiency), type and concentration of biochar amendment employed, as well as microbial community succession.