细胞外小泡
细胞内
纳米技术
细胞生物学
化学
计算生物学
细胞外
计算机科学
生物
材料科学
作者
Oskar Staufer,Franziska Dietrich,Rahul Rimal,Martin Schröter,Sebastian Fabritz,Heike Boehm,Smriti Singh,Martin Möller,Ilia Platzman,Joachim P. Spatz
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2021-09-03
卷期号:7 (36)
被引量:39
标识
DOI:10.1126/sciadv.abg6666
摘要
Extracellular vesicles (EVs) are fundamental for intercellular communication and influence nearly every process in cell physiology. However, because of their intricate molecular complexity, quantitative knowledge on their signaling mechanisms is missing, particularly impeding their therapeutic application. We used a complementary and quantitative engineering approach based on sequential synthetic bottom-up assembly of fully functional EVs with precisely controlled lipid, protein, and RNA composition. We show that the functionalities of synthetic EVs are analogous to natural EVs and demonstrate their programmable therapeutic administration for wound healing and neovascularization therapy. We apply transcriptome profiling to systematically decode synergistic effects between individual EV constituents, enabling analytical dissection and a fundamental understanding of EV signaling.
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