亚硝酸盐
电子受体
电子传输链
栅藻
叶绿素荧光
化学
光系统II
呼吸测定法
叶绿素
光合作用
环境化学
电缆管道
电子供体
叶绿素a
光化学
核化学
生物
生物化学
植物
藻类
材料科学
有机化学
润滑
催化作用
复合材料
硝酸盐
作者
S. Aparicio,Ángel Robles,J. Ferrer,A. Seco,L. Borrás
标识
DOI:10.1016/j.scitotenv.2021.152128
摘要
Total nitrite (TNO2 = HNO2 + NO-2) accumulation due to the activity of ammonia-oxidizing bacteria (AOB) was monitored in microalgae-bacteria consortia, and the inhibitory effect of nitrite/free nitrous acid (NO2-N/FNA) on microalgae photosynthesis and inhibition mechanism was studied. A culture of Scenedesmus was used to run two sets of batch reactors at different pH and TNO2 concentrations to evaluate the toxic potential of NO2-N and FNA. Photo-respirometric tests showed that NO2-N accumulation has a negative impact on net oxygen production rate (OPRNET). Chlorophyll a fluorescence analysis was used to examine the biochemical effects of NO2-N stress and the mechanism of NO2-N inhibition. The electron transport rate (ETR), non-photochemical quenching (NPQ), and JIP-test revealed that the electron transport chain between Photosystems II and I (PS II and PS I) was hindered at NO2-N concentrations above 25 g N m-3. Electron acceptor QA was not able to reoxidize and could not transfer electrons to the next electron acceptor, QB, accumulating P680+ (excited PS II reaction center) and limiting oxygen production. A semi-continuous reactor containing a Scenedesmus culture was monitored by photo-respirometry tests and Chlorophyll a fluorescence to calibrate NO2-N inhibition (5-35 g N m-3). Non-competitive inhibition and Hill-type models were compared to select the best-fitting inhibition equations. Inhibition was correctly modeled by the Hill-type model and a half inhibition constant (KI) for OPRNET, NPQ, maximum photosynthetic rate (ETRMAX) and the performance index PIABS was 23.7 ± 1.2, 26.36 ± 1.10, 39 ± 2 and 26.5 ± 0.4, respectively.
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