Recent Advances in Synthetic Notch Receptors for Biomedical Application

The synthetic Notch receptor has emerged as a potent tool for precisely modulating cellular functions. It constitutes a receptor system rooted in the Notch signaling pathway. SynNotch receptors, coupled with downstream transcription programs, hold promise for organoid and 3D tissue construction. Additionally, it enables the tracking and visualization of intercellular communication. Moreover, engineering SynNotch cells to carry specific receptors markedly enhances the efficacy and safety of immunotherapy. This review delineates the subdomains and tunable mechanisms of SynNotch, summarizing four core modes of combinatorial multiplexing potentially pivotal for regulating SynNotch cell functions. Furthermore, this review summarizes the multifaceted applications, advantages, and limitations of SynNotch, offering fresh insights into its future biomedical utilization.