自愈水凝胶
丝素
活力测定
化学
细胞包封
生物相容性
组织工程
体外
生物物理学
材料科学
生物医学工程
生物化学
丝绸
高分子化学
医学
生物
有机化学
复合材料
作者
Chavee Laomeephol,Helena Ferreira,Sorada Kanokpanont,Jittima Amie Luckanagul,Nuno M. Neves,Siriporn Damrongsakkul
出处
期刊:PubMed
日期:2022-01-01
卷期号:3 (3): 213-220
被引量:5
标识
DOI:10.12336/biomatertransl.2022.03.005
摘要
The tissue engineering triad comprises the combination of cells, scaffolds and biological factors. Therefore, we prepared cell- and drug-loaded hydrogels using in situ silk fibroin (SF) hydrogels induced by dimyristoyl glycerophosphoglycerol (DMPG). DMPG is reported to induce rapid hydrogel formation by SF, facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative capacity. In addition, DMPG can be used for liposome formulations in entrapping drug molecules. Dexamethasone (Dex) was loaded into the DMPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells, then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard conditions. Calcium production by cells cultured in DMPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing Dex. The extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic induction. The controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic medium. The results confirmed the possibility of using DMPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs.
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