Evaluation of Dasapushpagritham: An Ayurvedic formulation on L929 cell line through in vitro approach

Dasapushpagritham is a medicinal formulation for wound healing, mentioned in an ancient text called “Vishavaidhya-Jyostnika”. The major content of this formulation is Dasapushpam, which is a cluster of ten sacred plants and has significant medicinal uses as well as cultural importance in the state of Kerala, India. Each plant of this cluster is of great pharmaceutical value. The Dasapushpagritham contains the juice of all the ten plants (swarasam), paste of eleven other medicinal plants (kalkkam) and a ghee base. In this study the wound scratch assay is done with the Dasapushpagritham, kalkkam- Ghee-base and the aqueous extract of the ten plants. This was done to analyze the properties of the each separately. The scratch test was done using L929 (mouse fibroblast) cell lines. The plant extract showed the maximum wound closure than the Dasapushpagritham, and the base showed the least wound closure. From the result it was observed that the plant extract increases the cell proliferation but this property was reduced in the drug due to the presence of the base. GCMS analysis of the Dasapushpagritham was done to analyze the presence of various bioactive compounds. • Dasapushpa Ghritham formulation (contaning Dashapushpam whole plants) is usd for treating non-healing ulcers and wounds. • The study analyzed Dasapushpagritham, plant extract, kalkkam ghee base on L929cell line. • MTT assay, wound scratch assay and GCMS were done with the samples. • The plant extract shows cell proliferation and had wound closure values as that of the standard drug, kalkkam ghee base and Dasapushpagritham were slightly cytotoxic. • Gcms results proved the presence of angiogenic substances which is crucial for wound healing.