干细胞
细胞生物学
精子发生
生物
RNA剪接
生殖细胞
不育
体细胞
选择性拼接
基因
遗传学
内分泌学
外显子
核糖核酸
作者
Yujiao Wen,Shumin Zhou,Yiqian Gui,Zeqing Li,Lisha Yin,Wenchao Xu,Shenglei Feng,Xixiang Ma,Shiming Gan,Mengneng Xiong,Juan Dong,Keren Cheng,Xiaoli Wang,Shuiqiao Yuan
出处
期刊:Cell Reports
[Elsevier]
日期:2024-04-01
卷期号:43 (4): 114113-114113
标识
DOI:10.1016/j.celrep.2024.114113
摘要
Summary
The continuous regeneration of spermatogonial stem cells (SSCs) underpins spermatogenesis and lifelong male fertility, but the developmental origins of the SSC pool remain unclear. Here, we document that hnRNPU is essential for establishing the SSC pool. In male mice, conditional loss of hnRNPU in prospermatogonia (ProSG) arrests spermatogenesis and results in sterility. hnRNPU-deficient ProSG fails to differentiate and migrate to the basement membrane to establish SSC pool in infancy. Moreover, hnRNPU deletion leads to the accumulation of ProSG and disrupts the process of T1-ProSG to T2-ProSG transition. Single-cell transcriptional analyses reveal that germ cells are in a mitotically quiescent state and lose their unique identity upon hnRNPU depletion. We further show that hnRNPU could bind to Vrk1, Slx4, and Dazl transcripts that have been identified to suffer aberrant alternative splicing in hnRNPU-deficient testes. These observations offer important insights into SSC pool establishment and may have translational implications for male fertility.
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