Carboxymethyl chitosan coated alpha-linolenic acid nanoliposomes: Preparation, stability and release in vitro and in vivo

Nanoliposome encapsulation combined with carboxymethyl chitosan (CMCS) surface decoration was employed to improve physicochemical stability and oral bioavailability of alpha-linolenic acid (ALA). Different nanoliposome systems including ALA-loaded nanoliposomes (ALA-NLs) and CMCS-coated ALA-NLs (CMCS-ALA-NLs) were characterized through dynamic light scattering, transmission electron microscope, Fourier transform infrared spectroscopy and differential scanning calorimetry. The results showed that CMCS-ALA-NLs had good encapsulation efficiency of 79% and layer formation with nanosized spherical carrier. The physicochemical stability of CMCS-ALA-NLs was better than that of ALA-NLs. CMCS-ALA-NLs were able to regulate the release of ALA in a simulated gastrointestinal environment. In vivo testing found that ALA concentration of CMCS-ALA-NLs had an area under the curve of 1.32, which was 1.28 times higher than that of ALA-NLs and 2 times higher than that of ALA-emulsion. The absorption of ALA was improved by CMCS-ALA-NLs. It suggested that CMCS-coated nanoliposomes should be an available delivery strategy for transporting ALA.