Tetrahedral DNA nanostructure-corbelled click chemistry-based large-scale assembly of nanozymes for ratiometric fluorescence assay of DNA methyltransferase activity

We engineer DTN-corbelled click chemistry to trigger a HCR assembling a large-scale of nanozymes for ratiometric fluorescence detection of DNA Dam. The biosensor displayed a low detection limit of 0.001 U mL −1 for Dam MTase and was effective in real sample and methylation inhibitor screenings.