FTO‐mediated ZNF687 accelerates tumor growth, metastasis, and angiogenesis in colorectal cancer through the Wnt/β‐catenin pathway

血管生成 Wnt信号通路 基因敲除 癌症研究 转移 细胞生长 结直肠癌 生物 免疫印迹 癌症 细胞培养 信号转导 细胞生物学 基因 遗传学 生物化学
作者
Junyi Li,Shixin Li,Xiaoxiao Xing,Nini Liu,Suyu Lai,Daixiang Liao,Jun Li
出处
期刊:Biotechnology and Applied Biochemistry [Wiley]
卷期号:71 (2): 245-255
标识
DOI:10.1002/bab.2536
摘要

Abstract Colorectal cancer (CRC) is a common and lethal cancer. ZNF687 has been disclosed to take part in diversified cancers’ progression by serving as a facilitator. However, the detailed regulatory functions of ZNF687 in the CRC have not been investigated. This work is planned to probe the impacts of ZNF687 on CRC progression. The IHC, RT‐qPCR, and western blot assays were used to examine mRNA and protein gene expressions. The cell proliferation measurement was accompanied by a CCK‐8 assay. The Transwell assay was performed to evaluate cell invasion and migration. The angiogenesis ability was evaluated by a tube formation experiment. The m6A level was evaluated through MeRIP and m6A dot blot assays. The binding ability between ZNF687 and FTO (fat mass and obesity associated protein) was tested through an RIP assay. The β‐catenin nuclear translocation was assessed through an immunofluorescence assay. The tumor growth was evaluated through an in vivo assay. ZNF687 exhibited higher expression in CRC cells and resulted in a poor prognosis. Additionally, ZNF687 inhibition suppressed CRC cell proliferation, invasion, migration, and angiogenesis. Furthermore, the suppression of ZNF687 retarded the Wnt pathway. Through rescue assays, the reduced cell migration, proliferation, invasion, and angiogenesis mediated by ZNF687 knockdown could be reversed after BML‐284 (the activator of the Wnt pathway) treatment. Finally, it was explained that ZNF687 knockdown inhibited in vivo tumor growth. This study manifested that FTO‐mediated ZNF687 aggravated tumor growth, metastasis, and angiogenesis of CRC through Wnt/β‐catenin pathway. This finding may provide a hopeful molecular target for CRC treatment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
爆米花应助简单的依波采纳,获得10
1秒前
2021完成签到 ,获得积分10
2秒前
所所应助废物打工人采纳,获得10
2秒前
2秒前
呱呱乐完成签到,获得积分10
3秒前
rational发布了新的文献求助10
3秒前
觅云完成签到,获得积分10
3秒前
糟糕的冷雪完成签到,获得积分10
3秒前
hz发布了新的文献求助10
4秒前
沉默的钻石完成签到,获得积分10
4秒前
4秒前
全一斩完成签到,获得积分10
5秒前
夏远航完成签到,获得积分10
5秒前
7秒前
简.....完成签到,获得积分10
8秒前
吉祥应助一路前行采纳,获得20
8秒前
熊猫王666发布了新的文献求助10
8秒前
ChouNen完成签到,获得积分10
9秒前
abbyi完成签到,获得积分10
9秒前
9秒前
10秒前
10秒前
10秒前
酷炫小懒虫完成签到,获得积分10
11秒前
美丽的果汁完成签到 ,获得积分10
11秒前
魔幻的从蓉完成签到,获得积分10
11秒前
星星完成签到,获得积分10
11秒前
12秒前
12秒前
852应助abbyi采纳,获得10
13秒前
好困应助jiajiajia采纳,获得10
13秒前
揽星色应助jiajiajia采纳,获得10
13秒前
sss完成签到,获得积分10
13秒前
大萝贝发布了新的文献求助10
13秒前
Ava应助星际牛仔采纳,获得10
13秒前
打牙祭发布了新的文献求助10
13秒前
14秒前
14秒前
skippy发布了新的文献求助30
14秒前
Lxxx_7完成签到 ,获得积分10
14秒前
高分求助中
Sustainability in Tides Chemistry 1500
Handbook of the Mammals of the World – Volume 3: Primates 805
Gerard de Lairesse : an artist between stage and studio 500
Digging and Dealing in Eighteenth-Century Rome 500
Queer Politics in Times of New Authoritarianisms: Popular Culture in South Asia 500
Manual of Sewer Condition Classification 500
China's Relations With Japan 1945-83: The Role of Liao Chengzhi 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3068783
求助须知:如何正确求助?哪些是违规求助? 2722661
关于积分的说明 7478779
捐赠科研通 2369693
什么是DOI,文献DOI怎么找? 1256604
科研通“疑难数据库(出版商)”最低求助积分说明 609614
版权声明 596839