Preparation and characterization of infant formula concentrates: Simulation with human milk lipid composition and fat globule size and distribution

This study aims to prepare infant formula concentrates (IFs) simulating human milk fat globule (HMFG) composition, size and distribution. Mixed plant oil (21.3% palm oil, 2.8% flaxseed oil, 2.6% soybean oil, 29.9% rapeseed oil and 43.4% corn oil) and concentrated whey protein were applied to mimic triacylglycerols and phospholipids in HMFG respectively. IF1-IF6 were produced by different homogenization pressures or physically mixed with different proportions. IF4, prepared with 95% IF1 (4.29 μm), 1% IF2 (0.497 μm) and 4% IF3 (9.43 μm), showed a mean particle size of 3.96 μm and 49.77% milk fat globule membrane protein, and had the closest size distribution and interface with HMFG. The prepared IFs showed good stability with the Turbiscan stability index (IF6 (1.75)