检出限
同型
色谱法
化学
胶体金
单克隆抗体
分子生物学
抗体
生物
免疫学
纳米颗粒
纳米技术
材料科学
作者
Xinxin Xu,Lingling Guo,Aihong Wu,Liqiang Liu,Hua Kuang,Liguang Xu,Chuanlai Xu
标识
DOI:10.1080/19440049.2022.2120635
摘要
In this study, we developed a monoclonal antibody (mAb)-based colloidal gold immunochromatography assay (CG-ICA) strip for the rapid and sensitive detection of flubendiamide (FBD) in food samples. Our anti-FBD mAb 2B1 was highly specific and had a half-maximal inhibitory concentration (IC50) of 1.55 ng/mL under optimal conditions. It belongs to the IgG2a isotype and has a Kaff value of 1.52 × 109 mol/L. The strip provided a visual detection limit of 50 ng/g for both grapes and tomatoes, with a cut-off value of 1,000 ng/g and these qualitative results were observed within 15 min. For quantitative analysis, the calculated detection limits were 6 ng/g and 5 ng/g in grapes and tomatoes, respectively. The average recoveries of FBD ranged from 88.8% to 111.8% in grapes and 94.5% to 110.6% in tomatoes. The proposed strip assay is highly practical for screening FDB in food samples.
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