Chemically Different but Often Mistaken Phenolic Polymers of Food Plants: Proanthocyanidins and Lignin in Seeds

Flavonoid based proanthocyanidins and cinnamyl alcohol based lignins are chemically complex phenolic oligomers/polymers that are found in food plants. Although structurally very different, these two biopolymers are often not distinguished, for example, in the (quantitative) compositional analysis of cell walls and dietary fiber. Here, we analytically distinguish lignin and proanthocyanidins in dietary fiber samples by using degradative and nondegradative techniques and provide information about their occurrence, abundance, and structural characteristics in seeds of chokeberries, cranberries, raspberries, red currants, and grapes. These data revealed that the seeds of botanically diverse fruits largely differ in terms of their phenolic fiber polymers. The mostly hardened tissue of the seeds is not necessarily based on lignified cell walls. For example, red currant and chokeberry seeds almost exclusively contain proanthocyanidins, and raspberry seeds were clearly lignified (G-H-lignin) but did not contain proanthocyanidins. Our data also allows for estimating the bias of proanthocyanidins on different approaches of lignin analysis.