曙红
拉曼光谱
染色
H&E染色
荧光
化学
组织学
病理
材料科学
光学
医学
物理
作者
Sakiko Akaji,Takahiro Sagawa,Akira Honda,Natsuko Miyasaka,Kaori Sadakane,Takamichi Ichinose,Hirohisa Takano
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:147 (20): 4473-4479
被引量:1
摘要
Hematoxylin and eosin (HE) staining of tissue sections is a powerful tool for observing changes in the tissue structure and is used as the most fundamental and vital technique in histology. However, xenobiotics such as polymers and inorganic or organic materials have low dyeability, making it difficult to observe the distribution of materials across tissues. Raman spectroscopy is an advantageous technique for identifying materials in tissues using spectroscopic fingerprints by laser irradiation without staining. In this study, we developed a combined method for morphological observation and Raman spectral acquisition on the identical tissue slide by employing a decolorization step to remove eosin-induced fluorescence in HE-stained samples. Our method eliminated the fluorescence background and allowed the identical-field pathological observation, enabling simultaneous identification of biological responses and materials in tissues.
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