活性氧
秀丽隐杆线虫
氧化应激
突变体
生物
细胞生物学
线粒体
突变
线粒体ROS
基因敲除
基因
氧化磷酸化
遗传学
生物化学
作者
Atsuki En,Shuo Takanashi,Rena Okazaki,Michihiko Fujii
标识
DOI:10.1016/j.bbrc.2022.09.107
摘要
Oxygen is essential for aerobic organisms, but generates reactive oxygen species (ROS), which can cause cellular dysfunction by damaging cellular molecules. Many genes are involved in the regulation of ROS; however, much attention has not focused on them. To identify these genes, we screened for mutants with an altered sensitivity to oxidative stress in the nematode Caenorhabditis elegans. We isolated a novel mutant, oxy-7(qa5004) which showed an increased sensitivity to ROS in C. elegans. oxy-7 showed increased production of ROS and decreased longevity due to its increased oxidative stress. Genetic analysis revealed that oxy-7 has a causative mutation in Y71H2AM.9, a homologue of SLC30A9 which encodes a zinc transporter in mitochondria. We further showed that knockdown of human SLC30A9 caused increased ROS production in human cells as well. These results suggested an important role of mitochondrial zinc homeostasis in the regulation of ROS.
科研通智能强力驱动
Strongly Powered by AbleSci AI