Separation and purification as well as whitening efficacy of polysaccharide from Tricholoma matsutake Sing

Tricholoma matsutake polysaccharide extract was obtained from Tricholoma matsutake Sing with methods including water extraction and alcohol precipitation.Then the extract was preliminary separated and purified via protein removal with trichloroacetic acid treatment and fractional alcohol precipitation,and nine ingredients were obtained.Molecular weight,polysaccharide purity and whitening efficacy of the nine ingredients were determined and identified by gel permeation chromatography-multi-angle laser light scattering detector system and tyrosinase inhibition test.According to test result for purity and whitening efficacy,the best ingredient was chosen and further separated and purified with gel chromatography.Result indicated that the ingredient TMSP-6Ⅱ isolated from Tricholoma matsutake polysaccharide displays good tyrosinase inhibition activity.Its weight-average molecular weight was determined as 7.251×103 D and its purity was determined as higher than 95% with IC50 value determined as 136.4 mg·L-1.