男科
精子
体外受精
精液
医学
不育
人类受精
男性不育
精子活力
精子洗涤
精液分析
卵胞浆内精子注射
作者
Richard J. Paulson,Mark V. Sauer,Mary M. Francis,Thelma M. Macaso,Rogerio A. Lobo
标识
DOI:10.1016/s0015-0282(16)55262-5
摘要
Objective To evaluate sperm preincubation in tes and tris (TEST)-yolk buffer as a potential enhancing agent of fertilizing capacity of sperm during human in vitro fertilization (IVF). Design Oocytes obtained during IVF were divided into two groups: one group fertilized with TEST-yolk buffer-treated sperm and the other group with standard-prepared sperm. Setting The University of Southern California IVF Program. Patients Thirty-nine couples with suspected male factor infertility undergoing IVF. Interventions Preinsemination incubation of sperm in TEST-yolk buffer for 24hours. Main Outcome Measures Fertilization rates in vitro. Results TEST-yolk buffer-treated sperm fertilized 140 of 241 oocytes (58%), whereas control sperm fertilized 108 of 251 oocytes (43%). Of four couples who had previously failed to fertilize during IVF, one fertilized with both specimens and three fertilized with TEST-yolk buffer-treated sperm only. Conclusions TEST-yolk buffer pretreatment of sperm for 24hours results in higher fertilization rates during IVF among suspected male factor patients.
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