Phylogenetic, expression and functional characterizations of the maize NLP transcription factor family reveal a role in nitrate assimilation and signaling

拟南芥 生物 氮同化 基因 硝酸盐 异位表达 突变体 转录因子 转基因 转基因作物 遗传学 植物 生态学
作者
Zhangkui Wang,Lei Zhang,Ci Sun,Riliang Gu,Guohua Mi,Lixing Yuan
出处
期刊:Physiologia Plantarum [Wiley]
卷期号:163 (3): 269-281 被引量:21
标识
DOI:10.1111/ppl.12696
摘要

Although nitrate represents an important nitrogen (N) source for maize, a major crop of dryland areas, the molecular mechanisms of nitrate uptake and assimilation remain poorly understood. Here, we identified nine maize NIN-like protein (ZmNLP) genes and analyzed the function of one member, ZmNLP3.1, in nitrate nutrition and signaling. The NLP family genes were clustered into three clades in a phylogenic tree. Comparative genomic analysis showed that most ZmNLP genes had collinear relationships to the corresponding NLPs in rice, and that the expansion of the ZmNLP family resulted from segmental duplications in the maize genome. Quantitative PCR analysis revealed the expression of ZmNLP2.1, ZmNLP2.2, ZmNLP3.1, ZmNLP3.2, ZmNLP3.3, and ZmNLP3.4 was induced by nitrate in maize roots. The function of ZmNLP3.1 was investigated by overexpressing it in the Arabidopsis nlp7-1 mutant, which is defective in the AtNLP7 gene for nitrate signaling and assimilation. Ectopic expression of ZmNLP3.1 restored the N-deficient phenotypes of nlp7-1 under nitrate-replete conditions in terms of shoot biomass, root morphology and nitrate assimilation. Furthermore, the nitrate induction of NRT2.1, NIA1, and NiR1 gene expression was recovered in the 35S::ZmNLP3.1/nlp7-1 transgenic lines, indicating that ZmNLP3.1 plays essential roles in nitrate signaling. Taken together, these results suggest that ZmNLP3.1 plays an essential role in regulating nitrate signaling and assimilation processes, and represents a valuable candidate for developing transgenic maize cultivars with high N-use efficiency.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
SciGPT应助冉亦采纳,获得20
1秒前
领导范儿应助yanzw采纳,获得10
1秒前
灵巧南松发布了新的文献求助10
1秒前
xly发布了新的文献求助10
2秒前
科研通AI2S应助青1995采纳,获得10
2秒前
2秒前
路弈完成签到,获得积分10
3秒前
小钱全完成签到,获得积分10
4秒前
小陈完成签到,获得积分10
6秒前
顾青完成签到,获得积分10
7秒前
英俊的铭应助bqin采纳,获得10
7秒前
小钱全发布了新的文献求助10
7秒前
asd关闭了asd文献求助
7秒前
小马甲应助殷勤的采文采纳,获得10
9秒前
9秒前
jane123完成签到,获得积分10
9秒前
lz完成签到,获得积分20
10秒前
852应助爱思考的我采纳,获得30
11秒前
11秒前
Bean完成签到,获得积分10
11秒前
12秒前
12秒前
12秒前
自然馈赠发布了新的文献求助10
13秒前
13秒前
俭朴的雅彤完成签到,获得积分10
13秒前
14秒前
烂漫幻雪完成签到,获得积分10
15秒前
15秒前
河马卡卡完成签到,获得积分10
15秒前
冉亦发布了新的文献求助20
16秒前
17秒前
123发布了新的文献求助10
18秒前
hl发布了新的文献求助10
18秒前
小熊发布了新的文献求助10
19秒前
殷勤的采文完成签到,获得积分10
19秒前
鸣蜩十三完成签到,获得积分10
20秒前
qcl发布了新的文献求助10
20秒前
Akim应助河马卡卡采纳,获得10
20秒前
20秒前
高分求助中
Sustainability in ’Tides Chemistry 2000
Sustainability in ’Tides Chemistry 1500
The ACS Guide to Scholarly Communication 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Ethnicities: Media, Health, and Coping 800
Treatise on Geomorphology(2nd Edition - March 1, 2022) 520
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3070024
求助须知:如何正确求助?哪些是违规求助? 2724039
关于积分的说明 7483616
捐赠科研通 2371113
什么是DOI,文献DOI怎么找? 1257302
科研通“疑难数据库(出版商)”最低求助积分说明 609889
版权声明 596879