中国仓鼠卵巢细胞
二氢叶酸还原酶
转染
互补DNA
分子生物学
生物
质粒
细胞培养
仓鼠
基因组DNA
基因
遗传学
作者
Kazuya Onomichi,Yuzuru Eto,Hiroshiro Shibai
标识
DOI:10.1093/oxfordjournals.jbchem.a122023
摘要
Expression of human interleukin 2 (IL-2) at high levels has been achieved in Chinese hamster ovary (CHO) cells by amplification of transfected sequences. Plasmids containing the human IL-2 cDNA or genomic DNA and mouse dihydrofolate reductase (DHFR) cDNA were transfected into DHFR-negative CHO cells. Transformants expressing DHFR were selected in media lacking nucleosides, and cells which amplified both DHFR and IL-2 genes were obtained by exposure to increasing methotrexate (MTX) concentrations. These cell lines constitutively expressed elevated levels of IL-2 at a concentration of 2 mg/liter. These cell lines continued to produce IL-2 stably through at least 1 month, even in the absence of MTX.
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