Targeting a Monocyte Subset to Reduce Inflammation

炎症 单核细胞 生物 医学 免疫学
作者
Siamon Gordon
出处
期刊:Circulation Research [Ovid Technologies (Wolters Kluwer)]
卷期号:110 (12): 1546-1548 被引量:32
标识
DOI:10.1161/res.0b013e31825ec26d
摘要

HomeCirculation ResearchVol. 110, No. 12Targeting a Monocyte Subset to Reduce Inflammation Free AccessArticle CommentaryPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissionsDownload Articles + Supplements ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toSupplemental MaterialFree AccessArticle CommentaryPDF/EPUBTargeting a Monocyte Subset to Reduce Inflammation Siamon Gordon Siamon GordonSiamon Gordon From the Sir William Dunn School of Pathology, University of Oxford, Oxford, UK. Originally published8 Jun 2012https://doi.org/10.1161/RES.0b013e31825ec26dCirculation Research. 2012;110:1546–1548is corrected byCorrectionTherapeutic siRNA Silencing in Inflammatory Monocytes in Mice Leuschner et al Nat Biotech. 2011;29:1005–1010.Recently in Nature Biotechnology, Leuschner et al1 described an elegant method of limiting the recruitment of a proinflammatory subset of monocytes in mouse models of inflammation. Although providing prospects for therapeutic application, especially in cardiovascular diseases, it raises questions that make it necessary to proceed with caution.In this technical tour-de-force, Leuschner et al1 exploited the findings that murine macrophages that consist of two defined subsets differing in Ly6 antigen expression also differ in their expression of a major chemokine receptor, CCR2, responsible for the recruitment of circulating LY6C hi CCR2+ monocytes to peripheral sites of inflammation. They utilized lipid nanoparticles containing siRNA against CCR2 to deplete the recruitment of this subset and to attenuate inflammation in several models of vascular injury (myocardial infarction, atherosclerotic plaques), islet cell transplantation in diabetic mice, and tumor-associated macrophage recruitment. These striking results raise the possibility of limiting the deleterious effects of selected monocyte subsets while preserving housekeeping and clearance functions of resident tissue macrophages, which are essential for host homeostasis and survival. By optimizing the nanoparticle delivery system and the siRNA used for silencing, combined with sophisticated imaging in defined mouse models of disease, they achieved striking improvements over currently available anti-CCR2 antibodies or small molecule inhibitors.Figure summarizes the experimental strategy, which is based on previous knowledge of monocyte subpopulations and the importance of CCR2+ monocyte recruitment to sites of a range of metabolic and inflammatory stimuli. Inhibition of the inflammatory monocyte subset was demonstrated by gene ablation or reduction of CCR2 expression and antibody blockade of the actions of MCP-1, its ligand, in vitro and in vivo. The nanoparticles, which had been optimized for biocompatible delivery, localized rapidly to spleen, liver, and bone marrow after intravenous delivery; the encapsulated siRNA, selected for nuclease stability and reduced immunostimulation, effectively decreased CCR2 message and protein content, and reduced the levels of circulating LY6C hi monocytes and their migratory activity. They demonstrated partial selectivity for LY6 hi monocytes compared with LY6 lo monocytes and other phagocytes, polymorphonuclear leukocytes and myeloid dendritic cells, and, to a greater extent, for lymphocytes. The inflammatory disease models included acute ischemia reperfusion injury after coronary artery ligation, chronic inflammatory atherosclerosis plaque development in apolipoprotein E–deficient mice, survival of pancreatic allografts in streptozotocin-induced diabetes, and two transplantable tumors, a lymphoma and colorectal carcinoma. These experiments illustrate the role of CCR2+ monocyte depletion in limiting acute and chronic inflammatory lesions delivered in advance of lesion development or therapeutically after pathology had been established. The authors exploited and extended their previous discovery that murine splenic red pulp provides a major and important reservoir of monocytes for mobilization to injured organs.2Download figureDownload PowerPointFigure. Schematic representation of the strategy adopted to deliver siRNA nanoparticles in order to deplete LY6C hi CCR2+ inflammatory monocyte recruitment to an experimental cardiac infarct in mice. Note that CCR2 is also required for monocyte release from bone marrow. Figure kindly provided by Dr M. Nahrendorf.The goal of selective therapeutic modulation of monocyte/macrophage phenotype has become the "holy grail" of much contemporary research, not only in cardiovascular disease, where they are the major purveyors of inflammatory pathology, but also in obesity and malignancy, which they promote. Circulating monocytes in mice and humans occur as two well-characterized subsets, with differential expression of chemokine receptors (CX3CR1) as well as CCR2, and with differential expression of a range of other genes encoding receptors such as the FcR and CD16, as demonstrated by microarray analysis.3 Their life history and entry into tissues varies with differential recruitment in inflammation compared with the resident tissue macrophage populations present in the steady state. The patrolling subpopulation of monocytes remains intravascular, utilizing the adhesion molecule LFA-1 to perform poorly understood trophic functions for the endothelium.4 Recruitment of monocytes by microbial ligands can result in the acquisition of dendritic cell–associated properties such as expression of a surface lectin related to DC-SIGN. These properties are responsible for homing to T-cell areas and have the ability to capture and present antigens to lymphoid cells.5 Interconversion of macrophages and dendritic cells within tissues also has been described.6There is a clear distinction in the origin and distribution of inflammatory recruited monocytes and resident more sessile macrophage populations, which are constitutively present in tissues in the absence of overt inflammation. The recently recruited monocytes can differentiate rapidly into macrophages that display enhanced turnover and shorter survival times than resident macrophages. They adapt to the unique local microenvironments in different organs and alter their expression of adhesion and chemokine receptors that determine their localization; they can die by apoptosis or necrosis, or they can emigrate readily from sites of inflammation involving molecules, such as netrin, by poorly understood mechanisms.7Recent studies have highlighted the complexity of resident macrophage populations in different tissues. Epidermal Langerhans cells and microglia arise from yolk sac and may turn over locally, albeit at a low level, compared with bone marrow–derived mononuclear phagocytes.8 The abundant resident macrophage populations in tissues such as liver (Kupffer cells), gut, and lung (alveolar macrophages), as well as in lymphoid organs, are also phenotypically variable and distinct from inflammatory monocyte-derived cells. Once outside the vascular compartment, macrophages are modulated by the local connective tissue matrix, as well as cytokines and possible microbial products, and display considerable phenotypic heterogeneity. Their variability extends far beyond the simplistic classifications of M1 (proinflammatory, interferon γ–induced) and M2 (antiinflammatory, TH2-cytokine–induced) phenotypes associated with bacterial stimuli, cell-mediated immune activation and allergy, or parasitic infection.9What, then, are the implications of extensive monocyte/macrophage heterogeneity for selective targeting, and to what extent does the present study fall short of achieving the holy grail of "surgical" ablation of undesired functions? It will be considerably more difficult to access extravascular macrophages, although local delivery, eg, by the subcutaneous or intratracheal route, can be envisaged. This aspect could be a particular advantage in manipulating the cardiovascular system for therapeutic purposes. More problematic is the dynamic complexity, plasticity, and phenotypic variability of tissue macrophages, especially in relation to optimal recruitment and regulation of inflammatory cells to prevent persistent inflammation and to achieve controlled repair. If monocytic CCR2-dependent recruitment is hindered, then can other chemokine receptors or myeloid cells, including LY6C lo monocytes or polymorphonuclear leukocytes, compensate with attendant risks of tissue injury? Is antecedent inflammation not required for subsequent repair? This is suggested by the authors in a study of the role of extramedullary monocytopoiesis in infarct healing and accelerated evolution of heart failure after reduced inflammatory cell recruitment.10 Could the risk of atherosclerotic plaque vulnerability decrease but entail a greater risk of aneurysm? Above all, will the risk of infection be enhanced, along with inability of the host to mount a rapid and effective innate and acquired immune response? Inhibition of monocyte recruitment by antiCD11b/CD18 antibody in an autoimmune model of diabetes resulted in reduced lymphoid cell recruitment,11 which could be beneficial or detrimental to the host by recruiting regulatory or activatory subpopulations of T lymphocytes. Similarly, monocyte-derived dendritic cells can be tolerogenic or activatory and host-protective or pathogenic. In summary, the two-edged sword of immune-mediated host defense against injury is delicately balanced and may be tilted to pathological repair by monocyte depletion.Finally, humans and mice do express many differences as well as common features.3 The importance of a major splenic reservoir in humans requires further investigation. The authors suggest that the approach followed in regard to CCR2 can be extended to other myeloid and lymphoid populations. Nanoparticles provide a more concentrated delivery system, and targeting a particular monocyte subset could well be more efficient by removing a whole cell, rather than targeting a single molecule. Although promising, and perhaps more effective than present small molecule inhibitors and antibodies for therapy, these can be expected to improve in potency and specificity. However, cardiovascular disease may provide a particularly favorable field for targeting inflammation by the nanoparticle/siRNA strategy so elegantly developed here.AcknowledgmentsThe author is grateful to Frederic Geissmann and Simon Yona for useful discussions.FootnotesThe opinions expressed in this Commentary are not necessarily those of the editors or of the American Heart Association.Commentaries serve as a forum in which experts highlight and discuss articles (published elsewhere) that the editors of Circulation Research feel are of particular significance to cardiovascular medicine.Commentaries are edited by Aruni Bhatnagar and Ali J. Marian.Correspondence to Siamon Gordon, William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom. E-mail Siamon.[email protected]ox.ac.ukReferences1. Leuschner F, Dutta P, Gorbatov R , et al. Therapeutic siRNA silencing in inflammatory monocytes in mice. Nat Biotech. 2011; 29:1005–1010.CrossrefMedlineGoogle Scholar2. Swirski FK, Nahrendorf M, Etzrodt M, Wildgruber M, Cortez-Retamozo V, Panizzi P, Figueiredo JL, Kohler RH, Chudnovskiy A, Waterman P, Aikawa E, Mempel TR, Libby P, Weissleder R, Pittet MJ. Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science. 2009; 325:612–616.CrossrefMedlineGoogle Scholar3. Ingersoll MA, Spanbroek R, Lottaz C, Gautier EL, Frankenberger M, Hoffmann R, Lang R, Haniffa M, Collin M, Tacke F, Habenicht AJ, Ziegler-Heitbrock L, Randolph GJ. Comparison of gene expression profiles between human and mouse monocyte subsets. Blood. 2010; 115:e10–e19.CrossrefMedlineGoogle Scholar4. Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K. Development of monocytes, macrophages, and dendritic cells. Science. 2010; 327:656–661.CrossrefMedlineGoogle Scholar5. Cheong C, Matos I, Choi JH , et al. Microbial stimulation fully differentiates monocytes to DC-SIGN/CD209(+) dendritic cells for immune T cell areas. Cell. 2010; 143:416–429.CrossrefMedlineGoogle Scholar6. Rivollier A, He J, Kole A, Valatas V, Kelsall BL. Inflammation switches the differentiation program of LY6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon. J Exp Med. 2012; 209:139–155.CrossrefMedlineGoogle Scholar7. van Gils JM, Derby MC, Fernandes LR , et al. The neuroimmune guidance cue netrin-1 promotes atherosclerosis by inhibiting the emigration of macrophages from plaques. Nat Immun. 2012; 13:136–143.CrossrefGoogle Scholar8. Schulz C, Gomez Perdiguero E, Chorro L, Szabo-Rogers H, Cagnard N, Kierdorf K, Prinz M, Wu B, Jacobsen SE, Pollard JW, Frampton J, Liu KJ, Geissmann F. A lineage of myeloid cells independent of Myb and hemopoietic stem cells. Science.2012; 336:86–90.CrossrefMedlineGoogle Scholar9. Martinez FO, Helming L, Gordon S. Alternative activation of macrophages: an immunologic perspective. Annu Rev Immunol. 2009; 27:451–483.CrossrefMedlineGoogle Scholar10. Leuschner F, Rauch PJ, Ueno T , et al. Rapid monocyte kinetics in acute myocardial infarction are sustained by extramedullary monocytopoiesis. J Exp Med. 2012; 209:123–137.CrossrefMedlineGoogle Scholar11. Hutchings P, Rosen H, O'Reilly L, Simpson E, Gordon S, Cooke A. Transfer of diabetes in mice prevented by blockade of adhesion promoting receptor on macrophages. Nature. 1990; 348:639–642.CrossrefMedlineGoogle Scholar Previous Back to top Next FiguresReferencesRelatedDetailsCited By Park J, Kim J, Kim Y, Huang M, Chang J, Sim A, Jung H, Lee W, Hyun Y and Lee J (2021) Reparative System Arising from CCR2(+) Monocyte Conversion Attenuates Neuroinflammation Following Ischemic Stroke, Translational Stroke Research, 10.1007/s12975-020-00878-x, 12:5, (879-893), Online publication date: 1-Oct-2021. Li H, Chen C and Wang D (2021) Inflammatory Cytokines, Immune Cells, and Organ Interactions in Heart Failure, Frontiers in Physiology, 10.3389/fphys.2021.695047, 12 Park J, Chang J, Kim J and Lee J (2020) Monocyte Transmodulation: The Next Novel Therapeutic Approach in Overcoming Ischemic Stroke?, Frontiers in Neurology, 10.3389/fneur.2020.578003, 11 Wen Q, Zhao H, Yao W, Zhang Y, Fu H, Wang Y, Xu L, Zhang X, Kong Y and Huang X (2020) Monocyte subsets in bone marrow grafts may contribute to a low incidence of acute graft‐vs‐host disease for young donors, Journal of Cellular and Molecular Medicine, 10.1111/jcmm.15557, 24:16, (9204-9216), Online publication date: 1-Aug-2020. Kolenchukova O, Sarmatova N and Moshev A (2020) Phagocytic activity of blood monocytes in response to methicillin-resistant strains of Staphylococcus aureus, Russian Journal of Infection and Immunity, 10.15789/2220-7619-PAO-1181, 10:3, (551-557) Savchenko A, Borisov A, Kudryavtsev I and Moshev A (2020) Dependence of phenotype and chemiluminescent activity of monocytes on the Tregulatory cells content in patients with kidney cancer, Medical Immunology (Russia), 10.15789/1563-0625-DOP-1890, 22:2, (347-356) Parashar D, Rajendran V, Shukla R and Sistla R (2020) Lipid-based nanocarriers for delivery of small interfering RNA for therapeutic use, European Journal of Pharmaceutical Sciences, 10.1016/j.ejps.2019.105159, 142, (105159), Online publication date: 1-Jan-2020. Yonezawa S, Koide H and Asai T (2020) Recent advances in siRNA delivery mediated by lipid-based nanoparticles, Advanced Drug Delivery Reviews, 10.1016/j.addr.2020.07.022, 154-155, (64-78), . Savchenko A, Borisov A, Cherdancev D, Pervova O, Kudryavcev I and Belenyuk V (2018) REGULATORY INFLUENCE OF BLOOD MONOCYTES ON THE POPULATION COMPOSITION OF GRANULOCYTES AND THE STATE OF THEIR RESPIRATORY BURST IN THE WIDESPREAD PURULENT PERITONITIS, Russian Journal of Infection and Immunity, 10.15789/2220-7619-2018-2-201-210, 8:2, (201-210) Su Y, Chen C, Tsai N, Huang C, Wang H, Kung C, Lin W, Cheng B, Su C, Hsiao S and Lu C (2017) The Role of Monocyte Percentage in Osteoporosis in Male Rheumatic Diseases, American Journal of Men's Health, 10.1177/1557988317721642, 11:6, (1772-1780), Online publication date: 1-Nov-2017. Hu C, Meng X, Huang C, Shen C and Li J (2016) Frontline Science: ATF3 is responsible for the inhibition of TNF-α release and the impaired migration of acute ethanol-exposed monocytes and macrophages, Journal of Leukocyte Biology, 10.1189/jlb.2HI1115-491R, 101:3, (633-642), Online publication date: 1-Mar-2017. Zhang J, Peng K, Zhang J, Meng X, Ji F and Wang M (2017) Dexmedetomidine preconditioning may attenuate myocardial ischemia/reperfusion injury by down-regulating the HMGB1-TLR4-MyD88-NF-кB signaling pathway, PLOS ONE, 10.1371/journal.pone.0172006, 12:2, (e0172006) Gouwy M, Ruytinx P, Radice E, Claudi F, Van Raemdonck K, Bonecchi R, Locati M, Struyf S and Appel S (2016) CXCL4 and CXCL4L1 Differentially Affect Monocyte Survival and Dendritic Cell Differentiation and Phagocytosis, PLOS ONE, 10.1371/journal.pone.0166006, 11:11, (e0166006) Loukov D, Naidoo A, Puchta A, Marin J and Bowdish D (2016) Tumor necrosis factor drives increased splenic monopoiesis in old mice, Journal of Leukocyte Biology, 10.1189/jlb.3MA0915-433RR, 100:1, (121-129), Online publication date: 1-Jul-2016. Zhou L, Zhao G, Liu Q, Jiang S, Wang Y and Zhang D (2015) Silencing collapsin response mediator protein-2 reprograms macrophage phenotype and improves infarct healing in experimental myocardial infarction model, Journal of Inflammation, 10.1186/s12950-015-0053-8, 12:1, Online publication date: 1-Dec-2015. Leuschner F, Courties G, Dutta P, Mortensen L, Gorbatov R, Sena B, Novobrantseva T, Borodovsky A, Fitzgerald K, Koteliansky V, Iwamoto Y, Bohlender M, Meyer S, Lasitschka F, Meder B, Katus H, Lin C, Libby P, Swirski F, Anderson D, Weissleder R and Nahrendorf M (2014) Silencing of CCR2 in myocarditis, European Heart Journal, 10.1093/eurheartj/ehu225, 36:23, (1478-1488), Online publication date: 14-Jun-2015., Online publication date: 14-Jun-2015. Dal-Secco D, Wang J, Zeng Z, Kolaczkowska E, Wong C, Petri B, Ransohoff R, Charo I, Jenne C and Kubes P (2015) A dynamic spectrum of monocytes arising from the in situ reprogramming of CCR2+ monocytes at a site of sterile injury, Journal of Experimental Medicine, 10.1084/jem.20141539, 212:4, (447-456), Online publication date: 6-Apr-2015. Serhan C, Dalli J, Colas R, Winkler J and Chiang N (2015) Protectins and maresins: New pro-resolving families of mediators in acute inflammation and resolution bioactive metabolome, Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 10.1016/j.bbalip.2014.08.006, 1851:4, (397-413), Online publication date: 1-Apr-2015. Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X and Qin X (2015) Targeted 25-hydroxyvitamin D3 1α-hydroxylase Adoptive Gene Therapy Ameliorates DSS-induced Colitis Without Causing Hypercalcemia in Mice, Molecular Therapy, 10.1038/mt.2014.201, 23:2, (339-351), Online publication date: 1-Feb-2015. Aldo P, Racicot K, Craviero V, Guller S, Romero R and Mor G (2014) Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages, American Journal of Reproductive Immunology, 10.1111/aji.12288, 72:3, (270-284), Online publication date: 1-Sep-2014. Grainger J, Askenase M, Guimont-Desrochers F, Fonseca D and Belkaid Y (2014) Contextual functions of antigen-presenting cells in the gastrointestinal tract, Immunological Reviews, 10.1111/imr.12167, 259:1, (75-87), Online publication date: 1-May-2014. Zhou X, Luo Y, Ji W, Zhang L, Dong Y, Ge L, Lu R, Sun H, Guo Z, Yang G, Jiang T, Li Y and Zhang Z (2013) Modulation of Mononuclear Phagocyte Inflammatory Response by Liposome-Encapsulated Voltage Gated Sodium Channel Inhibitor Ameliorates Myocardial Ischemia/Reperfusion Injury in Rats, PLoS ONE, 10.1371/journal.pone.0074390, 8:9, (e74390) Cook J and Hagemann T (2013) Tumour-associated macrophages and cancer, Current Opinion in Pharmacology, 10.1016/j.coph.2013.05.017, 13:4, (595-601), Online publication date: 1-Aug-2013. Lugo-Villarino G and Neyrolles O (2013) Dressed not to kill: CD16 + monocytes impair immune defence against tuberculosis , European Journal of Immunology, 10.1002/eji.201243256, 43:2, (327-330), Online publication date: 1-Feb-2013. Related articlesCorrectionCirculation Research. 2012;111:e53-e53 June 8, 2012Vol 110, Issue 12 Advertisement Article InformationMetrics © 2012 American Heart Association, Inc.https://doi.org/10.1161/RES.0b013e31825ec26dPMID: 22679136 Originally publishedJune 8, 2012 PDF download Advertisement
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