Measurement of the respiration rate of fresh produce under different temperature is essential for the design of modiued atmosphere packaging (MAP). In this work respiration rate of fresh-cut bitter gourd was measured at storage temperatures of 8±2 and 30±2°C. As expected, temperature was the most inuuential factor on respiration rate. The respiration rate of pre-treated fresh-cut bitter gourd was studied under closed system. In closed system the respiration rate was less in 8±2°C with 59.64 ml/kg h (RO2) and 29 ml/kg h (RCO2) compared to ambient condition (30±2°C) of 87 ml/kg h -RO2, 40.6 ml/kg h -RCO2). As the temperature increases, the respiration rate also increases.