CO2-Responsive Spherical Polyelectrolyte Brush with Multi-Stimulation for Reversible Protein Immobilization and Release

Multi-responsive materials have received extensive interest in many areas due to their smart characteristics. This paper presents rationally designed multi-responsive spherical polyelectolyte brushes composed of a solid polystyrene (PS) core and a poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) shell synthesized by photoemulsion polymerization. Based on dynamic light scattering, Zeta potential, turbidity measurements, isothermal titration calorimetry, and UV-vis spectroscopy, PS-PDMAEMA works as a good potential adsorbent for bovine serum albumin (BSA) for which the maximum adsorption capability could reach up to 5190mg g−1. Moreover, the immobilization and release of protein on the polymer brush could be adjusted with different triggers, including the pH, ionic strength, and temperature. Furthermore, the green gas triggers, CO2 and N2, could be employed in the BSA@ PS-PDMAEMA system by easily bubbling over many cycles without any salt accumulation. The main reason for the observed actions is the brushes could be switched alternately between extended and collapsed states with different stimulations. Upon comparing the circular dichroism spectra of original and released BSA after many cycles of adsorption and release, it’s clear that the protein can retain its initial biological activity after release from the PS-PDMAEMA. This work provides an effective and green way to immobilize and release proteins in biotechnology.