芹菜素
脂多糖
小胶质细胞
化学
NF-κB
免疫印迹
药理学
信号转导
肿瘤坏死因子α
炎症
生物化学
免疫学
类黄酮
生物
抗氧化剂
基因
作者
Pengfei Chen,Xianhao Huo,Wenqing Liu,Kai Li,Zhipeng Sun,Jihui Tian
标识
DOI:10.1080/08923973.2019.1688345
摘要
Objective: Apigenin is a natural flavonoid compound extracted from Matricaria chamomilla. We evaluated the anti-inflammatory effects of apigenin in this study using the Lipopolysaccharide (LPS)-stimulated BV2 microglia.Methods: BV2 cells were treated with apigenin for 1 h and then treated with LPS. The inflammatory cytokine productions were tested by qRT-PCR and ELISA. The expression of GSK3β, Nrf2, and NF-κB signaling pathways were measured by western blot analysis.Results: Apigenin significantly attenuated LPS-induced TNF-α, IL-1β, and IL-6 production. Apigenin suppressed LPS-induced NF-κB activation. Furthermore, GSK3β, Nrf2, and HO-1 were concentration-dependently increased by apigenin. The suppression of apigenin on LPS-induced inflammatory response and NF-κB activation were prevented when Nrf2 was knocked out or by GSK3β inhibitor.Conclusions: Collectively, apigenin suppressed LPS-induced microglia activation via activating GSK3β/Nrf2 signaling pathway.
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