Rapid and highly sensitive portable detection of African swine fever virus

Transboundary and Emerging DiseasesVolume 68, Issue 2 p. 952-959 SHORT COMMUNICATION Rapid and highly sensitive portable detection of African swine fever virus Jade Daigle, Jade Daigle National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaSearch for more papers by this authorChukwunonso Onyilagha, Chukwunonso Onyilagha orcid.org/0000-0002-5592-7095 National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaSearch for more papers by this authorThang Truong, Thang Truong Canadian Science Centre for Human and Animal Health - National Microbiology Laboratory, Winnipeg, MB, CanadaSearch for more papers by this authorVan Phan Le, Van Phan Le College of Veterinary Medicine, Vietnam National University of Agriculture (VNUA), Trau Quy- Gia Lam- Hanoi, VietnamSearch for more papers by this authorBui Thi To Nga, Bui Thi To Nga College of Veterinary Medicine, Vietnam National University of Agriculture (VNUA), Trau Quy- Gia Lam- Hanoi, VietnamSearch for more papers by this authorThi Lan Nguyen, Thi Lan Nguyen College of Veterinary Medicine, Vietnam National University of Agriculture (VNUA), Trau Quy- Gia Lam- Hanoi, VietnamSearch for more papers by this authorAlfonso Clavijo, Alfonso Clavijo National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada USDA, Agricultural Research Service, Manhattan, KS, USASearch for more papers by this authorAruna Ambagala, Corresponding Author Aruna Ambagala aruna.ambagala@canada.ca orcid.org/0000-0003-1783-2105 National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada Department of Comparative Biology, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada Correspondence Aruna Ambagala, National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada. Email: aruna.ambagala@canada.caSearch for more papers by this author Jade Daigle, Jade Daigle National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaSearch for more papers by this authorChukwunonso Onyilagha, Chukwunonso Onyilagha orcid.org/0000-0002-5592-7095 National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, CanadaSearch for more papers by this authorThang Truong, Thang Truong Canadian Science Centre for Human and Animal Health - National Microbiology Laboratory, Winnipeg, MB, CanadaSearch for more papers by this authorVan Phan Le, Van Phan Le College of Veterinary Medicine, Vietnam National University of Agriculture (VNUA), Trau Quy- Gia Lam- Hanoi, VietnamSearch for more papers by this authorBui Thi To Nga, Bui Thi To Nga College of Veterinary Medicine, Vietnam National University of Agriculture (VNUA), Trau Quy- Gia Lam- Hanoi, VietnamSearch for more papers by this authorThi Lan Nguyen, Thi Lan Nguyen College of Veterinary Medicine, Vietnam National University of Agriculture (VNUA), Trau Quy- Gia Lam- Hanoi, VietnamSearch for more papers by this authorAlfonso Clavijo, Alfonso Clavijo National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada USDA, Agricultural Research Service, Manhattan, KS, USASearch for more papers by this authorAruna Ambagala, Corresponding Author Aruna Ambagala aruna.ambagala@canada.ca orcid.org/0000-0003-1783-2105 National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada Department of Comparative Biology, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada Correspondence Aruna Ambagala, National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada. Email: aruna.ambagala@canada.caSearch for more papers by this author First published: 06 August 2020 https://doi.org/10.1111/tbed.13770Citations: 9 Reproduced with the permission of the Minister of Agriculture. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract African swine fever (ASF) continues to spread across Asia, devastating pig populations. The disease is nearly 100% fatal in pigs, and currently, there is no effective vaccine available. Therefore, early detection of ASF is critical for effective disease control. The testing process usually requires samples to be shipped to a central laboratory, which may take many hours of travel or shipping time, delaying the results needed for a rapid response. The ability to confirm ASFV-infected animals on-site or in a regional laboratory that has limited technical capacity and/or infrastructure should eliminate these issues. This study describes the successful transfer of a highly sensitive and specific laboratory-validated real-time PCR assay to a portable pen-side thermocycler, which can be operated in the field for rapid detection of ASFV following a quick manual nucleic acid extraction from a wide array of clinical samples including aggregate samples such as oral fluids. The performance of the portable assay was comparable to the laboratory-based assay. The true portability of the assay was evaluated in seven ASF-suspected farms in Vietnam by testing eighty-nine freshly collected whole blood samples on-site. The results obtained on-site were in agreement with the laboratory data obtained the following day. Availability of this field-deployable molecular assay would eliminate the need to ship samples to a central laboratory, when rapid laboratory results are required, ultimately improving the response time. CONFLICT OF INTEREST None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper. Open Research DATA AVAILABILITY STATEMENT All data related to this manuscript will be made available upon request from the corresponding author. Citing Literature Supporting Information Filename Description tbed13770-sup-0001-TableS1.docxWord document, 18.1 KB Table S1 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume68, Issue2March 2021Pages 952-959 RelatedInformation