Antioxidants increase blastocyst cryosurvival and viability post-vitrification

玻璃化 胚泡 男科 胚胎 低温保存 胚胎移植 喉发生学 抗氧化剂 活力测定 氧化应激 化学 胚胎培养 生物 胚胎发生 细胞凋亡 生物化学 医学 细胞生物学
作者
Thi T. Truong,David K. Gardner
出处
期刊:Human Reproduction [Oxford University Press]
卷期号:35 (1): 12-23 被引量:48
标识
DOI:10.1093/humrep/dez243
摘要

What is the effect of antioxidants acetyl-L-carnitine, N-acetyl-L-cysteine and α-lipoic acid (A3) in vitrification and warming solutions on mouse blastocyst development and viability?The combination of three antioxidants in vitrification solutions resulted in mouse blastocysts with higher developmental potential in vitro and increased viability as assessed by both an outgrowth model in vitro and fetal development following uterine transfer.The antioxidant combination of acetyl-L-carnitine, N-acetyl-L-cysteine and α-lipoic acid present in IVF handling and embryo culture media has significant beneficial effects on mouse embryo and fetal development, especially under oxidative stress.The study was a laboratory-based analysis of an animal model. Rapid cooling through vitrification was conducted on F1 mouse blastocysts, with antioxidants (A3) supplemented in vitrification and/or warming solutions, followed by culture and embryo transfer.Pronucleate oocytes were collected and cultured in groups to Day 4 blastocysts. Expanded blastocysts were vitrified and warmed in solutions with and without the A3 antioxidants and cultured for a further 24 h. Blastocyst cell number and allocation, apoptosis and histone acetylation levels were all quantified, and viability through outgrowths and transfers assessed.Mouse blastocysts vitrified with no antioxidants had significantly lower cell numbers (P < 0.001) and higher apoptotic cells (P < 0.05) compared to non-vitrified embryos. Addition of combined A3 antioxidants to the vitrification and warming solutions resulted in a significant increase in inner cell mass cell (ICM) number (P < 0.001) and total cell number (P < 0.01), and an increase in outgrowth area (P < 0.05), which correlated with the increased fetal weight (P < 0.05), crown rump length (P < 0.05) and limb development (P < 0.05) determined following transfer compared to embryos with no antioxidants. Furthermore, while blastocyst vitrification significantly reduced acetylation levels (P < 0.05) compared to non-vitrified embryos, the inclusion of A3 antioxidants helped to ameliorate this.Embryo development was only examined in the mouse.Results in this study demonstrate that vitrification and warming of blastocysts have significant detrimental effects on embryo histone acetylation and subsequent viability. The presence of antioxidants in the vitrification solutions helps to alleviate the negative effects of cryopreservation. Our data indicate that antioxidants need to be present in the medium at the time of exposure to increased oxidative stress associated with vitrification and that prior exposure (i.e. during culture or IVF alone) is insufficient to protect cells against cryo-induced injury. Hence, A3 antioxidants may assist in maintaining the viability of vitrified human embryos in ART through the reduction of oxidative stress.This work was funded by a research grant from Vitrolife AB (Sweden). The authors have no conflict of interest to declare.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Zyk完成签到,获得积分10
刚刚
刚刚
1秒前
Aprilapple完成签到,获得积分10
1秒前
1秒前
c_123完成签到,获得积分10
2秒前
量子星尘发布了新的文献求助10
2秒前
456发布了新的文献求助10
3秒前
anna发布了新的文献求助10
4秒前
professorY完成签到 ,获得积分10
5秒前
wes完成签到 ,获得积分10
6秒前
6秒前
乐乐应助科研通管家采纳,获得10
7秒前
SciGPT应助科研通管家采纳,获得10
7秒前
CodeCraft应助科研通管家采纳,获得10
7秒前
乐乐应助科研通管家采纳,获得10
7秒前
cong完成签到 ,获得积分10
7秒前
SciGPT应助科研通管家采纳,获得10
7秒前
曹沛岚完成签到,获得积分10
7秒前
CodeCraft应助科研通管家采纳,获得10
7秒前
7秒前
7秒前
雪山飞龙发布了新的文献求助10
8秒前
英俊的铭应助c_123采纳,获得10
8秒前
马小跳发布了新的文献求助50
9秒前
9秒前
南风完成签到,获得积分10
10秒前
11秒前
12秒前
越啊发布了新的文献求助10
13秒前
研友_LX66qZ发布了新的文献求助10
13秒前
研友_ZzrWKZ完成签到 ,获得积分10
14秒前
如意闭月完成签到,获得积分10
20秒前
心静听炊烟完成签到 ,获得积分10
24秒前
yin景景给yin景景的求助进行了留言
26秒前
29秒前
小魏哥完成签到,获得积分10
33秒前
35秒前
35秒前
36秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989069
求助须知:如何正确求助?哪些是违规求助? 3531351
关于积分的说明 11253589
捐赠科研通 3269939
什么是DOI,文献DOI怎么找? 1804851
邀请新用户注册赠送积分活动 882074
科研通“疑难数据库(出版商)”最低求助积分说明 809073