作者
Xin Li,Ji Hyun Yang,Min Shen,Xinyu Xie,Guangjian Liu,Yaxi Xu,Feng‐Hua Lv,Hua Yang,Yonglin Yang,Changbin Liu,Ping Zhou,Pengcheng Wan,Yunsheng Zhang,Lei Gao,Jiahao Yang,Wen-Hui Pi,Yan-Ling Ren,Zhiqiang Shen,Zhen Wang,Juan Deng,Song-Song Xu,Hosein Salehian-Dehkordi,EEr Hehua,Ali Esmailizadeh,Mostafa Dehghani-Qanatqestani,Ondřej Štěpánek,Christina Weimann,G. Erhardt,Agraw Amane,Joram M. Mwacharo,Jianlin Han,Olivier Hanotte,Johannes A. Lenstra,Juha Kantanen,David W. Coltman,James W. Kijas,Michael William Bruford,P. Kathiravan,Xinhua Wang,Menghua Li
摘要
Abstract Understanding the genetic changes underlying phenotypic variation in sheep ( Ovis aries ) may facilitate our efforts towards further improvement. Here, we report the deep resequencing of 248 sheep including the wild ancestor ( O. orientalis ), landraces, and improved breeds. We explored the sheep variome and selection signatures. We detected genomic regions harboring genes associated with distinct morphological and agronomic traits, which may be past and potential future targets of domestication, breeding, and selection. Furthermore, we found non-synonymous mutations in a set of plausible candidate genes and significant differences in their allele frequency distributions across breeds. We identified PDGFD as a likely causal gene for fat deposition in the tails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses. Our results provide insights into the demographic history of sheep and a valuable genomic resource for future genetic studies and improved genome-assisted breeding of sheep and other domestic animals.