Aqueous extract of Polygonatum sibiricum ameliorates ethanol‐induced mice liver injury via regulation of the Nrf2/ARE pathway

Journal of Food BiochemistryVolume 45, Issue 1 e13537 FULL ARTICLE Aqueous extract of Polygonatum sibiricum ameliorates ethanol-induced mice liver injury via regulation of the Nrf2/ARE pathway Guangjun Wang, Guangjun Wang Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Resources, Software, Writing - original draft, Writing - review & editingSearch for more papers by this authorYouwei Fu, Youwei Fu Department of Health Education, Anhui Suzhou Center for Disease Control and Prevention, Suzhou Anhui, China Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Resources, Supervision, Validation, Writing - review & editingSearch for more papers by this authorJiujiu Li, Jiujiu Li Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Data curation, Formal analysis, ​Investigation, Software, Writing - review & editingSearch for more papers by this authorYanni Li, Yanni Li Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Data curation, Formal analysis, Software, VisualizationSearch for more papers by this authorQihong Zhao, Qihong Zhao Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Conceptualization, Data curation, ​Investigation, Methodology, Project administrationSearch for more papers by this authorAnla Hu, Anla Hu Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Conceptualization, ​Investigation, Methodology, Project administrationSearch for more papers by this authorChuande Xu, Chuande Xu Anhui Jingtian Food Co., Ltd., Chizhou, China Contribution: Conceptualization, Funding acquisition, ​Investigation, ResourcesSearch for more papers by this authorDongliang Shao, Dongliang Shao Anhui Guoke Testing Technology Co., Ltd., Hefei, China Contribution: Formal analysis, ​Investigation, Methodology, ResourcesSearch for more papers by this authorWenjun Chen, Corresponding Author Wenjun Chen chenwj71024@163.com orcid.org/0000-0002-2458-7040 Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Correspondence Wenjun Chen, Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei 230032, China. Email: chenwj71024@163.com Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, ​Investigation, Methodology, Project administration, Resources, Supervision, Writing - original draft, Writing - review & editingSearch for more papers by this author Guangjun Wang, Guangjun Wang Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Resources, Software, Writing - original draft, Writing - review & editingSearch for more papers by this authorYouwei Fu, Youwei Fu Department of Health Education, Anhui Suzhou Center for Disease Control and Prevention, Suzhou Anhui, China Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Resources, Supervision, Validation, Writing - review & editingSearch for more papers by this authorJiujiu Li, Jiujiu Li Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Data curation, Formal analysis, ​Investigation, Software, Writing - review & editingSearch for more papers by this authorYanni Li, Yanni Li Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Data curation, Formal analysis, Software, VisualizationSearch for more papers by this authorQihong Zhao, Qihong Zhao Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Conceptualization, Data curation, ​Investigation, Methodology, Project administrationSearch for more papers by this authorAnla Hu, Anla Hu Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Contribution: Conceptualization, ​Investigation, Methodology, Project administrationSearch for more papers by this authorChuande Xu, Chuande Xu Anhui Jingtian Food Co., Ltd., Chizhou, China Contribution: Conceptualization, Funding acquisition, ​Investigation, ResourcesSearch for more papers by this authorDongliang Shao, Dongliang Shao Anhui Guoke Testing Technology Co., Ltd., Hefei, China Contribution: Formal analysis, ​Investigation, Methodology, ResourcesSearch for more papers by this authorWenjun Chen, Corresponding Author Wenjun Chen chenwj71024@163.com orcid.org/0000-0002-2458-7040 Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, China Correspondence Wenjun Chen, Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei 230032, China. Email: chenwj71024@163.com Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, ​Investigation, Methodology, Project administration, Resources, Supervision, Writing - original draft, Writing - review & editingSearch for more papers by this author First published: 26 October 2020 https://doi.org/10.1111/jfbc.13537Citations: 2 Guangjun Wang and Youwei Fu contributed equally to this work. Funding informationThis study was supported by the Natural Science Research Project of Colleges and Universities of Department of Education Anhui Province (KJ2018A0677) Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract This study investigates the hepatoprotective effect of the aqueous extract of Polygonatum sibiricum (AEPS) against ethanol-induced oxidative stress and explores underlying mechanisms. AEPS was administered by gavage to ICR mice for 30 days. The experimental mice were fed a 5% (v/v) ethanol on last 10 days and followed by a single megadose of ethanol (5 g/kg) to induce ethanol-induced liver injury. Pretreatment with AEPS significantly suppressed the ethanol-induced elevation of aminotransferase activities, total bilirubin (TBIL) level, triglyceride level, and alleviated liver histopathological lesions. Meanwhile, AEPS reduced the level of oxidative stress in the liver and significantly suppressed the mRNA levels of NOX1, p67phox, gp91phox, and CYP2E1. Additionally, AEPS significantly increased the mRNA and protein levels of Nrf2 and its downstream antioxidant genes and promoted the nuclear translocation of Nrf2 in mice liver. Therefore, AEPS can effectively reduce ethanol-induced liver injury via regulation of the Nrf2/ARE pathway. Practical applications Alcohol abuse and alcoholism have become a serious public health problem worldwide. Since liver is the major organ of alcohol metabolism, the most impactful damage of alcohol occurs in the liver. Polygonatum sibiricum is a traditional Chinese galenical and it also can be used as food ingredients. Most studies have reported that polysaccharide, flavonoids and saponins are the main bioactive compounds in Polygonatum sibiricum which play important roles in anti-oxidation. AEPS is the aqueous extract of Polygonatum sibiricum and AEPS can protect the mice liver against ethanol-induced oxidative damage. Thus it can be potential antioxidants to product hepatoprotective food and the study also provides a theoretical basis for the development and application of food about Polygonatum sibiricum. Citing Literature Volume45, Issue1January 2021e13537 RelatedInformation