清脆的
逆转录酶
素数(序理论)
生物
小鼠白血病病毒
白血病
计算生物学
病毒学
遗传学
核糖核酸
组合数学
数学
基因
作者
Julian Grünewald,Bret R. Miller,Regan Szalay,Peter K. Cabeceiras,Christopher J. Woodilla,Eliza Jane B. Holtz,Karl Petri,J. Keith Joung
标识
DOI:10.1038/s41587-022-01473-1
摘要
The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing.
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