代谢组学
代谢组
生物
计算生物学
生物逆境
鉴定(生物学)
植物代谢
系统生物学
有害生物分析
代谢物分析
生物技术
生化工程
生态学
生物信息学
非生物胁迫
生物化学
植物
基因
核糖核酸
工程类
作者
Megha Kumari,Kalpesh Nath Yagnik,Vaishali Gupta,Indrakant K. Singh,Ravi Gupta,Praveen Kumar Verma,Archana Singh
摘要
Abstract The advancement of metabolomics has assisted in the identification of various bewildering characteristics of the biological system. Metabolomics is a standard approach, facilitating crucial aspects of system biology with absolute quantification of metabolites using minimum samples, based on liquid/gas chromatography, mass spectrometry and nuclear magnetic resonance. The metabolome profiling has narrowed the wide gaps of missing information and has enhanced the understanding of a wide spectrum of plant–environment interactions by highlighting the complex pathways regulating biochemical reactions and cellular physiology under a particular set of conditions. This high throughput technique also plays a prominent role in combined analyses of plant metabolomics and other omics datasets. Plant metabolomics has opened a wide paradigm of opportunities for developing stress‐tolerant plants, ensuring better food quality and quantity. However, despite advantageous methods and databases, the technique has a few limitations, such as ineffective 3D capturing of metabolites, low comprehensiveness, and lack of cell‐based sampling. In the future, an expansion of plant–pathogen and plant–pest response towards the metabolite architecture is necessary to understand the intricacies of plant defence against invaders, elucidation of metabolic pathway operational during defence and developing a direct correlation between metabolites and biotic stresses. Our aim is to provide an overview of metabolomics and its utilities for the identification of biomarkers or key metabolites associated with biotic stress, devising improved diagnostic methods to efficiently assess pest and pathogen attack and generating improved crop varieties with the help of combined application of analytical and molecular tools.
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