核梭杆菌
阿克曼西亚
微生物学
肠道菌群
失调
拟杆菌科
结肠炎
生物
某种肠道细菌
炎症性肠病
炎症
梭杆菌
细菌
拟杆菌
化学
医学
内科学
免疫学
遗传学
牙龈卟啉单胞菌
疾病
作者
Xuejun Zeng,Jiahui Li,Xin Wang,Ling Liu,Shiqi Shen,Nanyang Li,Zhouli Wang,Yahong Yuan,Tianli Yue
标识
DOI:10.1021/acs.jafc.3c08050
摘要
The aim of this study was to investigate the intervention effect of kefir supernatant (KS) on the initiation and progression of an ulcerative colitis (UC) murine model. We established an UC murine model by orally administrating with 109 CFUs of Fusobacterium nucleatum for 3 weeks and 3% dextran sulfate sodium (DSS) treatment in the third week. KS was used to intervene in this colitis model. Our results showed that KS supplementation ameliorated the symptoms, restrained the secretion of pro-inflammatory cytokines (TNF-α, IL-6, and IL-17F), promoted the release of anti-inflammatory cytokines (IL-4 and IL-10), and ameliorated oxidative stress. Furthermore, the increased number of goblet cells and upregulated expression of MUC2, occludin and claudin-1 indicated that the colon barrier was protected by KS. Additionally, KS supplementation mitigated gut microbiota dysbiosis in the UC murine model, leading to an increase in the abundance of Blautia and Akkermansia and a decrease in the level of Bacteroides. The altered gut microbiota also affected colon metabolism, with differential metabolites mainly associated with the biosynthesis of the l-arginine pathway. This study revealed that KS supplementation restored the community structure of gut microbiota, altered the biosynthesis of l-arginine, and thereby modulated the process of colonic inflammation.
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